BMRB Entry 25171

Title:
Solution structure of MLL-IBD complex
Deposition date:
2016-09-28
Original release date:
2014-12-01
Authors:
Cierpicki, Tomasz; Pollock, Jonathan; Murai, Marcelo
Citation:

Citation: Cierpicki, Tomasz; Pollock, Jonathan; Murai, Marcelo. "The same site on LEDGF IBD domain represents therapeutic target for MLL leukemia and HIV"  Blood 124, 3730-3737 (2014).
PubMed: 25305204

Assembly members:

Assembly members:
entity_1, polymer, 50 residues, Formula weight is not available
entity_2, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32a

Data sets:
Data typeCount
13C chemical shifts533
15N chemical shifts148
1H chemical shifts744

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 50 residues - Formula weight is not available

1   GLYPROALAMETALAPROGLYPHEASPALA
2   ALALEUGLNVALSERALAALAILEGLYTHR
3   ASNLEUARGARGPHEARGALAVALPHEGLY
4   GLUSERGLYGLYGLYGLYGLYSERGLYGLU
5   ASPGLUGLNPHELEUGLYPHEGLYSERASP

Entity 2, entity_2 114 residues - Formula weight is not available

1   GLUGLUVALARGVALARGGLYSERVALLYS
2   LYSVALGLULYSLYSARGGLUTHRSERMET
3   ASPSERARGLEUGLNARGILEHISALAGLU
4   ILELYSASNSERLEULYSILEASPASNLEU
5   ASPVALASNARGCYSILEGLUALALEUASP
6   GLULEUALASERLEUGLNVALTHRMETGLN
7   GLNALAGLNLYSHISTHRGLUMETILETHR
8   THRLEULYSLYSILEARGARGPHELYSVAL
9   SERGLNVALILEMETGLULYSSERTHRMET
10   LEUTYRASNLYSPHELYSASNMETPHELEU
11   VALGLYGLUGLYASPSERVALILETHRGLN
12   VALLEUASNLYS

Samples:

sample_1: entity_1 0.34 mM; entity_2 0.34 mM; potassium phosphate 50 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks