BMRB Entry 25173

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for N-terminal domain of NikR
Deposition date:
2014-08-25
Original release date:
2015-10-23
Authors:
Dobrovolska, Olena; Ciurli, Stefano
Citation:

Citation: Mazzei, Luca; Dobrovolska, Olena; Musiani, Francesco; Zambelli, Barbara; Ciurli, Stefano. "On the interaction of Helicobacter pylori NikR, a Ni(II)-responsive transcription factor, with the urease operator: in solution and in silico studies"  J. Biol. Inorg. Chem. 20, 1021-1037 (2015).
PubMed: 26204982

Assembly members:

Assembly members:
NikR_N, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: e-proteobacteria   Taxonomy ID: 210   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Helicobacter pylori

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts143
15N chemical shifts49
1H chemical shifts48

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminal dopmain of NikR1

Entities:

Entity 1, N-terminal dopmain of NikR 64 residues - Formula weight is not available

1   GLYSERHISMETASPTHRPROASNLYSASP
2   ASPSERILEILEARGPHESERVALSERLEU
3   GLNGLNASNLEULEUASPGLULEUASPASN
4   ARGILEILELYSASNGLYTYRSERSERARG
5   SERGLULEUVALARGASPMETILEARGGLU
6   LYSLEUVALGLUASPASNTRPALAGLUASP
7   ASNPROASNASP

Samples:

sample_1: NikR_N, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O 10%; sodium chloride 0.15 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAJ55866 BAJ56159 BAJ58846 BAJ60367 BAM97196
EMBL CAX30022 CBI65698
GB AAD06843 AAD08380 ABL95954 ACD48784 ACI28034
REF NP_208130 WP_000354193 WP_000379491 WP_000379492 WP_000379493
SP B2UVA2 B5Z8Y5 O25896 Q9ZJP7
AlphaFold O25896 B2UVA2 B5Z8Y5 Q9ZJP7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks