BMRB Entry 25217

Name: 1H, 13C, and 15N Chemical Shift Assignments for AUX/IAA17
Published: 2014-12-15

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PDB ID: 2muk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25217
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for AUX/IAA17

Deposition date:

2014-09-11

Original release date:

2014-12-15

Authors:

Suh, Jeong-Yong; Han, Mookyoung

Citation:

Citation: Han, Mookyoung; Park, Yangshin; Kim, Iktae; Kim, Eun-Hee; Yu, Tae-Kyung; Rhee, Sangkee; Suh, Jeong-Yong. "Structural basis for the auxin-induced transcriptional regulation by Aux/IAA17" Proc. Natl. Acad. Sci. U. S. A. 111, 18613-18618 (2014).
PubMed: 25512488

Assembly members:

Assembly members:
Aux-IAA17, polymer, 113 residues, 12801.768 Da.

Natural source:

Natural source: Common Name: thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Aux-IAA17: GGPEAAAFVKVSMDGAPYLR KIDLRMYKSYDELSNALSNM FSSFTMGKHGGEEGMIDFMN ERKLMDLVNSWDYVPSYENK DGNWMLVGDVPWPMFVDTCK RLRLMKGSDAIGL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	602
13C chemical shifts	428
15N chemical shifts	108

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AUX IAA17	1

Entities:

Entity 1, AUX IAA17 113 residues - 12801.768 Da.

1

GLY

PRO

GLU

ALA

PHE

VAL

LYS

2

VAL

SER

MET

ASP

GLY

ALA

PRO

TYR

LEU

ARG

3

LYS

ILE

ASP

LEU

ARG

MET

TYR

LYS

SER

TYR

4

ASP

GLU

LEU

SER

ASN

ALA

LEU

SER

ASN

MET

5

PHE

SER

PHE

THR

MET

GLY

LYS

HIS

GLY

6

GLY

GLU

GLY

MET

ILE

ASP

PHE

MET

ASN

7

GLU

ARG

LYS

LEU

MET

ASP

LEU

VAL

ASN

SER

8

TRP

ASP

TYR

VAL

PRO

SER

TYR

GLU

ASN

LYS

9

ASP

GLY

ASN

TRP

MET

LEU

VAL

GLY

ASP

VAL

10

PRO

TRP

PRO

MET

PHE

VAL

ASP

THR

CYS

LYS

11

ARG

LEU

ARG

LEU

MET

LYS

GLY

SER

ASP

ALA

12

ILE

GLY

LEU

Samples:

sample_1: Aux-IAA17, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 10 mM; DTT 10 mM; H2O 90%; D2O 10%

sample_conditions_1: temperature: 298 K; pH: 7.4; pressure: 1 atm; ionic strength: 0.01 M

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Bruker Avance 900 MHz
Bruker Avance 600 MHz

PDB	2MUK
GB	AAB70451 AAB84354 AAC39439 AAC39440 AAG53997
REF	NP_171921 XP_002889499
SP	P93830
AlphaFold	P93830