BMRB Entry 25327

Title:
Bd0108
Deposition date:
2014-11-11
Original release date:
2014-12-17
Authors:
Prehna, Gerd
Citation:

Citation: Prehna, Gerd; Ramirez, Benjamin; Lovering, Andrew. "The Lifestyle Switch Protein Bd0108 of Bdellovibrio bacteriovorus Is an Intrinsically Disordered Protein"  PLoS One 9, e115390-e115390 (2014).
PubMed: 25514156

Assembly members:

Assembly members:
Bd0108, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: d-proteobacteria   Taxonomy ID: 959   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bdellovibrio bacteriovorus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET26b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts271
1H chemical shifts288
15N chemical shifts69

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Bd01081

Entities:

Entity 1, Bd0108 83 residues - Formula weight is not available

1   GLYSERHISMETALAASPGLUASNALAASN
2   ARGPROVALASNPROGLYGLUASPPROASN
3   GLUALAPHEARGSERTHRPROPHEGLUALA
4   THRTHRSERALALEUGLYASPCYSARGGLU
5   CYSILEALATYRARGTHRGLYALATHRTHR
6   GLYLYSGLYSERARGARGHISASPASPTHR
7   VALSERARGGLUILELYSGLYSERSERALA
8   THRPROGLYGLYSERGLULYSALAGLYTHR
9   GLYARGGLN

Samples:

sample_1: Bd0108, [U-99% 13C; U-99% 15N], 1 mM; TRIS 20 mM; sodium chloride 100 mM; beta-mercaptoethanol 2 mM; H2O 95%; D2O 5%

sample_conditions: temperature: 298 K; pH: 7.0; pressure: 1 atm; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions
3D HNCACBsample_1isotropicsample_conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions
3D HNCOsample_1isotropicsample_conditions
3D HCACOsample_1isotropicsample_conditions
3D HNHAsample_1isotropicsample_conditions
3D H(CCO)NHsample_1isotropicsample_conditions
3D HCCH-TOCSYsample_1isotropicsample_conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis, chemical shift assignment

TALOS, Cornilescu, Delaglio and Bax - data analysis

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks