BMRB Entry 25365

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for drosophila histone mRNA stem-loop-binding protein
Deposition date:
2014-11-24
Original release date:
2014-12-09
Authors:
Zhang, Jun; DeRose, Eugene; Hall, Traci
Citation:

Citation: Zhang, Jun; Tan, Dazhi; DeRose, Eugene; Perera, Lalith; Dominski, Zbigniew; Marzluff, William; Tong, Liang; Hall, Traci. "Molecular mechanisms for the regulation of histone mRNA stem-loop binding protein by phosphorylation"  Proc. Natl. Acad. Sci. U.S.A. 111, E2937-E2946 (2014).
PubMed: 25002523

Assembly members:

Assembly members:
SLBP, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PSMT3

Data sets:
Data typeCount
13C chemical shifts92
15N chemical shifts37
1H chemical shifts37

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SLBP1

Entities:

Entity 1, SLBP 93 residues - Formula weight is not available

1   SERSERSERTYRTHRGLUALAASPPROALA
2   ILELEUSERARGARGGLNLYSGLNILEASP
3   TYRGLYLYSASNTHRALAALATYRGLUARG
4   TYRVALGLUMETVALPROLYSASPGLUARG
5   THRARGASPHISPROARGGLUPROASNLYS
6   TYRGLYLYSTYRSERARGARGALAPHEASP
7   GLYLEUVALLYSILETRPARGLYSSERLEU
8   HISILETYRASPPROPROTHRGLNALAARG
9   ASPTHRALALYSASPGLUASNGLUASPGLU
10   ASPGLUASP

Samples:

sample_1: SLBP, [U-100% 13C; U-100% 15N], 0.5 mM; Hepes 20 mM; NaCl 500 mM

sample_conditions_1: ionic strength: 500 mM; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks