BMRB Entry 25391

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25391

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Title:
1H, 15N and 13C resonance assignments of translationally-controlled tumor protein from Nannochloropsis oceanica
Deposition date:
2014-12-11
Original release date:
2015-06-04
Authors:
Yao, Xingzhe; Feng, Yingang
Citation:

Citation: Yao, Xingzhe; Xiao, Yan; Cui, Qiu; Feng, Yingang. "1H, 15N and 13C resonance assignments of translationally-controlled tumor protein from photosynthetic microalga Nannochloropsis oceanica"  Biomol. NMR Assign. 9, 325-328 (2015).
PubMed: 25680850

Assembly members:

Assembly members:
NoTCTP, polymer, 188 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Nannochloropsis oceanica   Taxonomy ID: 145522   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Nannochloropsis oceanica

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NoTCTP: MIVYKDVISGDEVVSDALKI TPVMEGGEEVPGLFEVDSAM VAVGGGDIDIGCGNAFGGAG DDEGADDATQKENNVSGPSS FAYTAMPFSSKGEFKSWVKD YVRNVRQALKGSGVAVEDIK KFMEEAPTFVKWLVDKYDDL EFFMSKSMNPDAGLIFSYYK EGAHCPTFVYVKSGYKVVKF LEHHHHHH

Data sets:
Data typeCount
13C chemical shifts709
15N chemical shifts165
1H chemical shifts1103

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tumor protein monomer1

Entities:

Entity 1, tumor protein monomer 188 residues - Formula weight is not available

1   METILEVALTYRLYSASPVALILESERGLY
2   ASPGLUVALVALSERASPALALEULYSILE
3   THRPROVALMETGLUGLYGLYGLUGLUVAL
4   PROGLYLEUPHEGLUVALASPSERALAMET
5   VALALAVALGLYGLYGLYASPILEASPILE
6   GLYCYSGLYASNALAPHEGLYGLYALAGLY
7   ASPASPGLUGLYALAASPASPALATHRGLN
8   LYSGLUASNASNVALSERGLYPROSERSER
9   PHEALATYRTHRALAMETPROPHESERSER
10   LYSGLYGLUPHELYSSERTRPVALLYSASP
11   TYRVALARGASNVALARGGLNALALEULYS
12   GLYSERGLYVALALAVALGLUASPILELYS
13   LYSPHEMETGLUGLUALAPROTHRPHEVAL
14   LYSTRPLEUVALASPLYSTYRASPASPLEU
15   GLUPHEPHEMETSERLYSSERMETASNPRO
16   ASPALAGLYLEUILEPHESERTYRTYRLYS
17   GLUGLYALAHISCYSPROTHRPHEVALTYR
18   VALLYSSERGLYTYRLYSVALVALLYSPHE
19   LEUGLUHISHISHISHISHISHIS

Samples:

sample_1: NoTCTP, [U-13C; U-15N], 1 mM; sodium phosphate 50 mM; sodium chloride 200 mM; DTT 5 mM; EDTA 5 mM; sodium azide 0.02 % w/v; DSS 0.01 % w/v

sample_conditions_1: ionic strength: 250 mM; pH: 7.8; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HBHANHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D CCH-COSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks