BMRB Entry 25401

Title:
1H, 15N 13C Chemical shift assignments for CCL28
Deposition date:
2014-12-19
Original release date:
2015-01-20
Authors:
Thomas, Monica; Peterson, Francis; Volkman, Brian
Citation:

Citation: Thomas, Monica; Buelow, Becky; Nevins, Amanda; Jones, Stephanie; Peterson, Francis; Gundry, Rebekah; Grayson, Mitchell; Volkman, Brian. "Structure-function analysis of CCL28 in post viral asthma"  J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
CCL28, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Data sets:
Data typeCount
13C chemical shifts434
1H chemical shifts698
15N chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CCL281

Entities:

Entity 1, CCL28 108 residues - Formula weight is not available

1   SERGLUALAILELEUPROILEALASERSER
2   CYSCYSTHRGLUVALSERHISHISILESER
3   ARGARGLEULEUGLUARGVALASNMETCYS
4   ARGILEGLNARGALAASPGLYASPCYSASP
5   LEUALAALAVALILELEUHISVALLYSARG
6   ARGARGILECYSVALSERPROHISASNHIS
7   THRVALLYSGLNTRPMETLYSVALGLNALA
8   ALALYSLYSASNGLYLYSGLYASNVALCYS
9   HISARGLYSLYSHISHISGLYLYSARGASN
10   SERASNARGALAHISGLNGLYLYSHISGLU
11   THRTYRGLYHISLYSTHRPROTYR

Samples:

sample_1: CCL28, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 20 mM; sodium azide 0.02%; D2O, [U-99% 2H], 10%

sample_conditions_1: temperature: 298 K; pH: 6.2; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - data analysis, chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

DBJ BAG35056 BAJ20648
GB AAF87205 AAG16691 AAG43193 AAH62668 AAH69532
REF NP_001288802 NP_001288803 NP_001288804 NP_683513 XP_001135733
SP Q9NRJ3
AlphaFold Q9NRJ3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks