BMRB Entry 25418

Title:
Human Cytochrome c WT ferric form (oxidized)
Deposition date:
2015-01-07
Original release date:
2015-10-07
Authors:
Karsisiotis, Andreas Ioannis; Worrall, Jonathan; Deacon, Oliver
Citation:

Citation: Karsisiotis, Andreas Ioannis; Deacon, Oliver; Rajagopal, Badri; Macdonald, Colin; Blumenschein, Tharin; Moore, Geoffrey; Worrall, Jonathan. "Backbone resonance assignments of ferric human cytochrome c and the pro-apoptotic G41S mutant in the ferric and ferrous states"  Biomol. NMR Assign. 9, 415-419 (2015).
PubMed: 26123826

Assembly members:

Assembly members:
hCyt_c_WT, polymer, 104 residues, Formula weight is not available
HEME C, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pUC19Amp

Data sets:
Data typeCount
13C chemical shifts193
15N chemical shifts99
1H chemical shifts101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human Cyt c1
2human Cyt c2

Entities:

Entity 1, human Cyt c 104 residues - Formula weight is not available

1   GLYASPVALGLULYSGLYLYSLYSILEPHE
2   ILEMETLYSCYSSERGLNCYSHISTHRVAL
3   GLULYSGLYGLYLYSHISLYSTHRGLYPRO
4   ASNLEUHISGLYLEUPHEGLYARGLYSTHR
5   GLYGLNALAPROGLYTYRSERTYRTHRALA
6   ALAASNLYSASNLYSGLYILEILETRPGLY
7   GLUASPTHRLEUMETGLUTYRLEUGLUASN
8   PROLYSLYSTYRILEPROGLYTHRLYSMET
9   ILEPHEVALGLYILELYSLYSLYSGLUGLU
10   ARGALAASPLEUILEALATYRLEULYSLYS
11   ALATHRASNGLU

Entity 2, human Cyt c - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

HCC_WT_293K: sodium phosphate 20 mM; sodium chloride 50 mM; hCyt c WT, [U-100% 15N], 1 mM; HEME C 1 mM; H2O 90%; D2O, U-2H, 10%

293K: ionic strength: 70 mM; pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCHCC_WT_293Kisotropic293K
3D HNCACBHCC_WT_293Kisotropic293K
3D CBCA(CO)NHHCC_WT_293Kisotropic293K

Software:

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - chemical shift assignment, data analysis, processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker UltraShield Plus 800 MHz

Related Database Links:

BMRB 25420 25422
PDB
DBJ BAG34778 BAH24118
EMBL CAD28485 CAG46972 CAH89483 CAL38091 CAL38301
GB AAA35732 AAH05299 AAH08475 AAH08477 AAH09578
PRF 630485A
REF NP_001065289 NP_001124639 NP_061820 XP_001110791 XP_001140708
SP P00002 P99998 P99999 Q52V08 Q5RFH4
AlphaFold Q52V08 P99999 P00002 P99998 Q5RFH4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks