BMRB Entry 25447

Title:
solution Structure of KstB-PCP in kosinostatin biosynthesis
Deposition date:
2015-01-20
Original release date:
2016-01-19
Authors:
Zhao, Bin
Citation:

Citation: Zhao, Bin. "1H and 15N Assigned Chemical Shifts for KstB-PCP"  .

Assembly members:

Assembly members:
entity, polymer, 91 residues, 9865.176 Da.

Natural source:

Natural source:   Common Name: Micromonospora sp. TP-A0468   Taxonomy ID: 1182970   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Micromonospora Micromonospora sp. TP-A0468

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts273
15N chemical shifts92
1H chemical shifts615

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 91 residues - 9865.176 Da.

1   SERGLUMETGLNHISALASERVALILEALA
2   GLNPHEVALVALGLUGLUPHELEUPROASP
3   VALALAPROALAASPVALASPVALASPLEU
4   ASPLEUVALASPASNGLYVALILEASPALA
5   LEUGLYLEULEULYSVALILEALATRPLEU
6   GLUASPARGPHEGLYILEALAALAASPASP
7   VALGLULEUSERPROGLUHISPHEARGSER
8   ILEARGSERILEASPALAPHEVALVALGLY
9   ALATHRTHRPROPROVALGLUALALYSLEU
10   GLN

Samples:

sample_1: KstB-PCP(apo), [U-99% 13C; U-99% 15N], 2 mM; phosphate 80 mM; Sodium chloride 50 mM

sample_conditions_1: pH: 7.0

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

  • Agilent INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks