BMRB Entry 25455

Title:
Backbone, side chain and heme resonance assignments of cytochrome OmcF from Geobacter sulfurreducens
Deposition date:
2015-01-27
Original release date:
2016-11-02
Authors:
Dantas, Joana; Silva e Sousa, Marta; Salgueiro, Carlos; Bruix, Marta
Citation:

Citation: Dantas, Joana; Silva e Sousa, Marta; Salgueiro, Carlos; Bruix, Marta. "Backbone, side chain and heme resonance assignments of cytochrome OmcF from Geobacter sulfurreducens"  Biomol. NMR Assign. 9, 365-368 (2015).
PubMed: 25939275

Assembly members:

Assembly members:
OmcF_Polypeptide, polymer, 85 residues, Formula weight is not available
PROTOPORPHYRIN IX CONTAINING FE, non-polymer, 616.487 Da.

Natural source:

Natural source:   Common Name: Geobacter sulfurreducens   Taxonomy ID: 35554   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Geobacter sulfurreducens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pLBM4

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts235
15N chemical shifts86
1H chemical shifts557

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1OmcF1
2HEM2

Entities:

Entity 1, OmcF 85 residues - Formula weight is not available

1   SERGLYGLYSERGLYALAGLYGLYGLYGLU
2   LEUPHEALATHRHISCYSALAGLYCYSHIS
3   PROGLNGLYGLYASNTHRVALHISPROGLU
4   LYSTHRLEUALAARGALAARGARGGLUALA
5   ASNGLYILEARGTHRVALARGASPVALALA
6   ALATYRILEARGASNPROGLYPROGLYMET
7   PROALAPHEGLYGLUALAMETILEPROPRO
8   ALAASPALALEULYSILEGLYGLUTYRVAL
9   VALALASERPHEPRO

Entity 2, HEM - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: OmcF_Polypeptide, [U-100% 13C; U-100% 15N], 2 mM; PROTOPORPHYRIN IX CONTAINING FE 2 mM; H2O 90%; D2O, U-2H, 10%; sodium phosphate 45 mM; sodium chloride 100 mM

sample_2: OmcF_Polypeptide 2 mM; PROTOPORPHYRIN IX CONTAINING FE 2 mM; H2O 90%; D2O, U-2H, 10%; sodium phosphate 45 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 5; pressure: 1 atm; temperature: 308 K

sample_conditions_2: ionic strength: 100 mM; pH: 5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_2

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks