BMRB Entry 25557

Title:
Backbone chemical shift assignments for reduced peroxiredoxin Q from the plant pathogen Xanthomonas campestris.
Deposition date:
2015-03-28
Original release date:
2015-05-08
Authors:
Buchko, Garry; Perkins, Arden; Karplus, P.
Citation:

Citation: Buchko, Garry; Perkins, Arden; Parsonage, Derek; Poole, Leslie; Karplus, P.. "Backbone chemical shift assignments for Xanthomonas campestris peroxiredoxin Q in the reduced and oxidized states: a dramatic change in backbone dynamics"  Biomol. NMR Assign. 10, 57-61 (2016).
PubMed: 26438558

Assembly members:

Assembly members:
PrxQ, polymer, 160 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Xanthomonas campestris   Taxonomy ID: 339   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Xanthomonas campestris

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTHC

Data sets:
Data typeCount
13C chemical shifts504
15N chemical shifts151
1H chemical shifts151

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PrxQ1

Entities:

Entity 1, PrxQ 160 residues - Formula weight is not available

1   METTHRASPALAVALLEUGLULEUPROALA
2   ALATHRPHEASPLEUPROLEUSERLEUSER
3   GLYGLYTHRGLNTHRTHRLEUARGALAHIS
4   ALAGLYHISTRPLEUVALILETYRPHETYR
5   PROLYSASPSERTHRPROGLYCYSTHRTHR
6   GLUGLYLEUASPPHEASNALALEULEUPRO
7   GLUPHEASPLYSALAGLYALALYSILELEU
8   GLYVALSERARGASPSERVALLYSSERHIS
9   ASPASNPHECYSALALYSGLNGLYPHEALA
10   PHEPROLEUVALSERASPGLYASPGLUALA
11   LEUCYSARGALAPHEASPVALILELYSGLU
12   LYSASNMETTYRGLYLYSGLNVALLEUGLY
13   ILEGLUARGSERTHRPHELEULEUSERPRO
14   GLUGLYGLNVALVALGLNALATRPARGLYS
15   VALLYSVALALAGLYHISALAASPALAVAL
16   LEUALAALALEULYSALAHISALALYSGLN

Samples:

sample_1: PrxQ, [U-99% 13C; U-99% 15N], 1.0 ± 0.2 mM; sodium chloride 100 ± 2 mM; TRIS 20 ± 0.5 mM; DTT 1 ± 0.2 mM; H2O 93%; D2O, [U-2H], 7%

sample_conditions_1: ionic strength: 120 mM; pH: 7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1

Software:

SPARKY v3.115, Goddard - data analysis

Felix v2007, Accelrys Software Inc. - processing

NMR spectrometers:

  • Varian VNMRS 750 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks