BMRB Entry 25646

Title:
Backbone chemical shift assignment of rat p75NTR transmembrane and intracellular domains in lipid/protein nanodiscs
Deposition date:
2015-06-03
Original release date:
2015-09-16
Authors:
Mineev, Konstantin; Goncharuk, Sergey; Arseniev, Alexander
Citation:

Citation: Mineev, Konstantin; Goncharuk, Sergey; Kuzmichev, Pavel; Vilar, Marcal; Arseniev, Alexander. "NMR Dynamics of Transmembrane and Intracellular Domains of p75NTR in Lipid-Protein Nanodiscs"  Biophys. J. 109, 772-782 (2015).
PubMed: 26287629

Assembly members:

Assembly members:
p75-TMICD, polymer, 199 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEMEX-1

Data sets:
Data typeCount
1H chemical shifts468

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p75-TMICD1

Entities:

Entity 1, p75-TMICD 199 residues - Formula weight is not available

Sequence numbering corresponds to the Uniprot ID P07174

1   METHISHISHISHISHISHISGLYSERGLY
2   SERGLYLEUVALPROARGGLYSERTHRARG
3   GLYTHRTHRASPASNLEUILEPROVALTYR
4   CYSSERILELEUALAALAVALVALVALGLY
5   LEUVALALATYRILEALAPHELYSARGTRP
6   ASNSERCYSLYSGLNASNLYSGLNGLYALA
7   ASNSERARGPROVALASNGLNTHRPROPRO
8   PROGLUGLYGLULYSLEUHISSERASPSER
9   GLYILESERVALASPSERGLNSERLEUHIS
10   ASPGLNGLNTHRHISTHRGLNTHRALASER
11   GLYGLNALALEULYSGLYASPGLYASNLEU
12   TYRSERSERLEUPROLEUTHRLYSARGGLU
13   GLUVALGLULYSLEULEUASNGLYASPTHR
14   TRPARGHISLEUALAGLYGLULEUGLYTYR
15   GLNPROGLUHISILEASPSERPHETHRHIS
16   GLUALACYSPROVALARGALALEULEUALA
17   SERTRPGLYALAGLNASPSERALATHRLEU
18   ASPALALEULEUALAALALEUARGARGILE
19   GLNARGALAASPILEVALGLUSERLEUCYS
20   SERGLUSERTHRALATHRSERPROVAL

Samples:

sample_1: p75-TMICD, [U-99% 13C; U-99% 15N], 0.2 mM; MSP1 0.4 mM; DMPC 19.2 mM; DMPG 4.8 mM; potassium phosphate 20 mM; sodium chloride 50 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 70 mM; pH: 6.7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CARA v1.8, Keller and Wuthrich - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

UNP P07174
DBJ BAC29775
EMBL CAA28783
GB AAD17943 AAH38365 EDL15985 EDM05760
PRF 1303336A
REF NP_036742 NP_150086
SP P07174 Q9Z0W1
AlphaFold P07174 P07174 Q9Z0W1