BMRB Entry 25673

Title:
NMR structure for a 3-stranded parallel beta-sheet
Deposition date:
2015-06-24
Original release date:
2015-10-26
Authors:
Kung, Vanessa
Citation:

Citation: Kung, Vanessa; Cornilescu, Gabriel; Gellman, Samuel. "Impact of Strand Number on Parallel beta-Sheet Stability"  Angew. Chem. Int. Ed. Engl. 54, 14336-14339 (2015).
PubMed: 26457984

Assembly members:

Assembly members:
entity_1, polymer, 25 residues, 2629.136 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XERFYEKXXVQKFIRVXGVT IREKX

Data sets:
Data typeCount
1H chemical shifts172

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 25 residues - 2629.136 Da.

1   ACEGLUARGPHETYRGLULYS4G6DPRVAL
2   GLNLYSPHEILEARGVAL4FUGLYVALTHR
3   ILEARGGLULYSNH2

Samples:

sample_1: entity_1 2 mM; acetic acid 2.5 mM

sample_conditions_1: pH: 3.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H ROESYsample_1isotropicsample_conditions_1

Software:

X-PLOR_NIH v2.35, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz