BMRB Entry 25773

Title:
1H, 13C, 15N assignments of Trappin-2
Deposition date:
2015-08-26
Original release date:
2016-03-03
Authors:
LOTH, Karine; Abou Ibrahim Alami, Soha; Habes, Chahrazed; Zani, Marie-Louise; Delmas, Agnes; Moreau, Thierry; Landon, Celine
Citation:

Citation: Loth, Karine; Abou Ibrahim Alami, Soha; Habes, Chahrazed; Zani, Marie-Louise; Delmas, Agnes; Moreau, Thierry; Landon, Celine. "Complete assignment of 15N, 13C Trappin-2 and 1H assignment of its two domains, Elafin and Cementoin."  Biomol. NMR Assign. 10, 223-226 (2016).
PubMed: 26878852

Assembly members:

Assembly members:
Trappin-2, polymer, 95 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris   Vector: pPIC9

Data sets:
Data typeCount
13C chemical shifts401
15N chemical shifts95
1H chemical shifts652

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Trappin-21

Entities:

Entity 1, Trappin-2 95 residues - Formula weight is not available

1   ALAVALTHRGLYVALPROVALLYSGLYGLN
2   ASPTHRVALLYSGLYARGVALPROPHEASN
3   GLYGLNASPPROVALLYSGLYGLNVALSER
4   VALLYSGLYGLNASPLYSVALLYSALAGLN
5   GLUPROVALLYSGLYPROVALSERTHRLYS
6   PROGLYSERCYSPROILEILELEUILEARG
7   CYSALAMETLEUASNPROPROASNARGCYS
8   LEULYSASPTHRASPCYSPROGLYILELYS
9   LYSCYSCYSGLUGLYSERCYSGLYMETALA
10   CYSPHEVALPROGLN

Samples:

sample_1: Trappin-2, [U-100% 13C; U-100% 15N], 0.3 mM; sodium phosphate 25 mM; sodium chloride 300 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 300 mM; pH: 6.1; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNHAHBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

Ccpnmr, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks