BMRB Entry 25819

Title:
Solution Structure of Leptospiral LigA4 Big Domain
Deposition date:
2015-10-19
Original release date:
2015-10-19
Authors:
Mei, Song
Citation:

Citation: Mei, Song; Zhang, Jiahai; Zhang, Xuecheng; Tu, Xiaoming. "Solution Structure of Leptospiral LigA4 Big Domain"  Biochem. Biophys. Res. Commun. 467, 288-292 (2015).
PubMed: 26449456

Assembly members:

Assembly members:
entity, polymer, 92 residues, 9481.341 Da.

Natural source:

Natural source:   Common Name: Leptospira   Taxonomy ID: 171   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Leptospira not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet22

Data sets:
Data typeCount
13C chemical shifts233
15N chemical shifts100
1H chemical shifts457

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 92 residues - 9481.341 Da.

1   METPROALAALALEUVALSERILESERVAL
2   SERPROTHRASNSERTHRVALALALYSGLY
3   LEUGLNGLUASNPHELYSALATHRGLYILE
4   PHETHRASPASNSERASNSERASPILETHR
5   ASPGLNVALTHRTRPASPSERSERASNTHR
6   ASPILELEUSERILESERASNALASERASP
7   SERHISGLYLEUALASERTHRLEUASNGLN
8   GLYASNVALLYSVALTHRALASERILEGLY
9   GLYILEGLNGLYSERTHRASPPHELYSVAL
10   THRGLN

Samples:

sample_1: entity, [U-13C; U-15N], 0.5 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker AMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks