BMRB Entry 25836

Title:
NMR structure of OtTx1a - ICK
Deposition date:
2015-10-05
Original release date:
2016-10-13
Authors:
Nadezhdin, Kirill; Romanovskaya, Daria; Sachkova, Maria; Vassilevski, Alexander; Grishin, Evgeny; Kovalchuk, Sergey; Arseniev, Alexander
Citation:

Citation: Nadezhdin, Kirill; Romanovskaya, Daria; Sachkova, Maria; Vassilevski, Alexander; Grishin, Evgeny; Kovalchuk, Sergey; Kovalchuk, Evgeny. "NMR structure of OtTx1a - AMP in DPC micelles"  .

Assembly members:

Assembly members:
entity, polymer, 59 residues, 6373.258 Da.

Natural source:

Natural source:   Common Name: spiders   Taxonomy ID: 666126   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oxyopes Takobius

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-32a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts130
15N chemical shifts55
1H chemical shifts290

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 59 residues - 6373.258 Da.

1   GLYTHRPROVALGLYASNASNLYSCYSTRP
2   ALAILEGLYTHRTHRCYSSERASPASPCYS
3   ASPCYSCYSPROGLUHISHISCYSHISCYS
4   PROALAGLYLYSTRPLEUPROGLYLEUPHE
5   ARGCYSTHRCYSGLNVALTHRGLUSERASP
6   LYSVALASNLYSCYSPROPROALAGLU

Samples:

sample_1: entity, [U-100% 15N], 0.6 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CARA, Keller and Wuthrich - chemical shift assignment

Molmol, Koradi, Billeter and Wuthrich - structure solution

TOPSPIN, Bruker Biospin - collection, data analysis, processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

UNP P86716
AlphaFold S4TYR3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks