BMRB Entry 25857

Title:
NMR Solution Structure of Lacticin Q and Aureocin A53
Deposition date:
2015-10-22
Original release date:
2016-02-15
Authors:
Acedo, Jeella; van Belkum, Marco; Lohans, Christopher; Towle, Kaitlyn; Miskolzie, Mark; Vederas, John
Citation:

Citation: Acedo, Jeella; van Belkum, Marco; Lohans, Christopher; Towle, Kaitlyn; Miskolzie, Mark; Vederas, John. "Nuclear Magnetic Resonance Solution Structures of Lacticin Q and Aureocin A53 Reveal a Structural Motif Conserved among Leaderless Bacteriocins with Broad-Spectrum Activity"  Biochemistry 55, 733-742 (2016).
PubMed: 26771761

Assembly members:

Assembly members:
Aureocin_A53, polymer, 51 residues, 5994.296 Da.

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: Eubacteria   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Aureocin_A53: MSWLNFLKYIAKYGKKAVSA AWKYKGKVLEWLNVGPTLEW VWQKLKKIAGL

Data sets:
Data typeCount
1H chemical shifts403

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Aureocin A531

Entities:

Entity 1, Aureocin A53 51 residues - 5994.296 Da.

1   METSERTRPLEUASNPHELEULYSTYRILE
2   ALALYSTYRGLYLYSLYSALAVALSERALA
3   ALATRPLYSTYRLYSGLYLYSVALLEUGLU
4   TRPLEUASNVALGLYPROTHRLEUGLUTRP
5   VALTRPGLNLYSLEULYSLYSILEALAGLY
6   LEU

Samples:

sample_1: Aureocin A53 1 mM; DSS 0.01 % w/v; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

NMR spectrometers:

  • Varian vNMRJ 700 MHz