BMRB Entry 25900

Title:
NRAS Isoform 5
Deposition date:
2015-11-13
Original release date:
2016-03-21
Authors:
Markowitz, Joseph; Mal, Tapas; Yuan, Chunhua; Courtney, Nicholas; Patel, Mitra; Stiff, Andrew; Blachly, James; Walker, Christopher; Eisfeld, Ann-Kathrin; de la Chapelle, Albert; Carson, William
Citation:

Citation: Markowitz, Joseph; Mal, Tapas; Yuan, Chunhua; Courtney, Nicholas; Patel, Mitra; Stiff, Andrew; Blachly, James; Walker, Christopher; Eisfeld, Ann-Kathrin; de la Chapelle, Albert; Carson, William. "Structural Characterization of NRAS Isoform 5"  Protein Sci. 25, 1069-1074 (2016).
PubMed: 26947772

Assembly members:

Assembly members:
entity, polymer, 20 residues, 2120.511 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MTEYKLVVVGAGGVGKSHVW

Data sets:
Data typeCount
1H chemical shifts150

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 20 residues - 2120.511 Da.

1   METTHRGLUTYRLYSLEUVALVALVALGLY
2   ALAGLYGLYVALGLYLYSSERHISVALTRP

Samples:

sample_1: TFE, [U-99% 2H], 56%; entity 1 mM; sodium azide 10 uM; sodium phosphate 4 mM; EDTA, [U-99% 2H], 50 uM

sample_conditions_1: ionic strength: 0.004 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 13C-1H HSQC-TOCSYsample_1isotropicsample_conditions_1
2D 13C-1H HSQC-NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz