BMRB Entry 26050

Title:
STRUCTURE OF DK17 IN POPC:POPG:CHOLESTEROL:GM1 LUVS
Deposition date:
2016-05-11
Original release date:
2017-03-16
Authors:
Bera, Swapna; Bhunia, Anirban
Citation:

Citation: Bera, Swapna; Kar, Rajiv; Mondal, Susanta; Pahan, Kalipada; Bhunia, Anirban. "Structural Elucidation of the Cell-Penetrating Penetratin Peptide in Model Membranes at the Atomic Level: Probing Hydrophobic Interactions in the Blood-Brain Barrier"  Biochemistry 55, 4982-4996 (2016).
PubMed: 27532224

Assembly members:

Assembly members:
CELL_PENETRATING_PEPTIDES, polymer, 17 residues, 2368.891 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CELL_PENETRATING_PEPTIDES: DRQIKIWFQNRRMKWKK

Data sets:
Data typeCount
1H chemical shifts116

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DK17 IN POPC:POPG:CHOLESTEROL:GM1 LUVS1

Entities:

Entity 1, DK17 IN POPC:POPG:CHOLESTEROL:GM1 LUVS 17 residues - 2368.891 Da.

1   ASPARGGLNILELYSILETRPPHEGLNASN
2   ARGARGMETLYSTRPLYSLYS

Samples:

DK17_MIXEDLIPIDS: CELL PENETRATING PEPTIDES 0.5 mM; H2O 90%; D2O 10%; DSS 0.5 mM

sample_conditions_1: ionic strength: 10 mM; pH: 4.5; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYDK17_MIXEDLIPIDSisotropicsample_conditions_1
2D 1H-1H NOESYDK17_MIXEDLIPIDSisotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz