BMRB Entry 26509

Title:
Backbone resonance assignments and secondary structure determination for the human PHF6-ePHD1
Deposition date:
2015-02-14
Original release date:
2015-04-10
Authors:
Bao, Yun; Liu, Zhonghua; Zhang, Jiahai; Wu, Jihui; Shi, Yunyu
Citation:

Citation: Bao, Yun; Liu, Zhonghua; Zhang, Jiahai; Wu, Jihui; Shi, Yunyu. "1H, 13C and 15N resonance assignments and secondary structure of the human PHF6-ePHD1 domain"  Biomol. NMR Assign. 10, 1-4 (2016).
PubMed: 26286319

Assembly members:

Assembly members:
PHF6-ePHD1, polymer, 127 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T-1

Data sets:
Data typeCount
13C chemical shifts464
15N chemical shifts127
1H chemical shifts726

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PHF6-ePHD11

Entities:

Entity 1, PHF6-ePHD1 127 residues - Formula weight is not available

1   THRARGGLNARGLYSCYSGLYPHECYSLYS
2   SERASNARGASPLYSGLUCYSGLYGLNLEU
3   LEUILESERGLUASNGLNLYSVALALAALA
4   HISHISLYSCYSMETLEUPHESERSERALA
5   LEUVALSERSERHISSERASPASNGLUSER
6   LEUGLYGLYPHESERILEGLUASPVALGLN
7   LYSGLUILELYSARGGLYTHRLYSLEUMET
8   CYSSERLEUCYSHISCYSPROGLYALATHR
9   ILEGLYCYSASPVALLYSTHRCYSHISARG
10   THRTYRHISTYRHISCYSALALEUHISASP
11   LYSALAGLNILEARGGLULYSPROSERGLN
12   GLYILETYRMETVALTYRCYSARGLYSHIS
13   LYSLYSTHRALAHISASNSER

Samples:

sample_1: PHF6-ePHD1, [U-13C; U-15N], 0.6 mM; phosphate sodium 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 289 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Zhengrong and Bax - processing

NMR spectrometers:

  • Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks