BMRB Entry 26531

Title:
Human Eosinophil-Derived Neurotoxin
Deposition date:
2015-03-08
Original release date:
2015-04-10
Authors:
Gagne, Donald; Charest, Laurie-Anne; Doucet, Nicolas
Citation:

Citation: Gagne, Donald; Narayanan, Chitra; Bafna, Khushboo; Charest, Laurie-Anne; Agarwal, Pratul; Doucet, Nicolas. "1H, 13C, and 15N backbone resonance assignment of the human eosinophil-derived neurotoxin"  Biomol. NMR Assignments 11, 143-149 (2017).
PubMed: 28271277

Assembly members:

Assembly members:
EDN, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 562   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts261
15N chemical shifts113
1H chemical shifts113

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EDN1

Entities:

Entity 1, EDN 134 residues - Formula weight is not available

1   LYSPROPROGLNPHETHRTRPALAGLNTRP
2   PHEGLUTHRGLNHISILEASNMETTHRSER
3   GLNGLNCYSTHRASNALAMETGLNVALILE
4   ASNASNTYRGLNARGARGCYSLYSASNGLN
5   ASNTHRPHELEULEUTHRTHRPHEALAASN
6   VALVALASNVALCYSGLYASNPROASNMET
7   THRCYSPROSERASNLYSTHRARGLYSASN
8   CYSHISHISSERGLYSERGLNVALPROLEU
9   ILEHISCYSASNLEUTHRTHRPROSERPRO
10   GLNASNILESERASNCYSARGTYRALAGLN
11   THRPROALAASNMETPHETYRILEVALALA
12   CYSASPASNARGASPGLNARGARGASPPRO
13   PROGLNTYRPROVALVALPROVALHISLEU
14   ASPARGILEILE

Samples:

sample_1: EDN, [U-13C; U-15N], mM; ammonium acetate, [U-100% 15N], 7.5 mM; Glucose, [U-100% 13C], 10 % w/v; sodium acetate 15 mM

sample_conditions_1: ionic strength: 15 mM; pH: 5.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D CC(CO)NHsample_1isotropicsample_conditions_1
2D HSQCsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

CcpNmr_Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 800 MHz

Related Database Links:

UNP P10153
AlphaFold Q9UCG7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks