BMRB Entry 26631

Title:
SPOP_MATH_PUC complex
Deposition date:
2015-08-10
Original release date:
2016-01-05
Authors:
Grace, Christy; Pierce, Wendy; Mittag, Tanja
Citation:

Citation: Pierce, Wendy; Grace, Christy; Lee, Jihun; Nourse, Amanda; Marzahn, Melissa; Watson, Edmond; High, Anthony; Peng, Junmin; Schulman, Brenda; Mittag, Tanja. "Multiple weak linear motifs enhance recruitment and processivity in SPOP-mediated substrate ubiquitination"  J. Mol. Biol. 428, 1256-1271 (2016).
PubMed: 26475525

Assembly members:

Assembly members:
MATH-PUC_complex, polymer, 159 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BL21(DE3)

Data sets:
Data typeCount
13C chemical shifts375
15N chemical shifts128
1H chemical shifts130

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MATH1
2PUC1

Entities:

Entity 1, MATH 159 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSVALVAL
2   LYSPHESERTYRMETTRPTHRILEASNASN
3   PHESERPHECYSARGGLUGLUMETGLYGLU
4   VALILELYSSERSERTHRPHESERSERGLY
5   ALAASNASPLYSLEULYSTRPCYSLEUARG
6   VALASNPROLYSGLYLEUASPGLUGLUSER
7   LYSASPTYRLEUSERLEUTYRLEULEULEU
8   VALSERCYSPROLYSSERGLUVALARGALA
9   LYSPHELYSPHESERILELEUASNALALYS
10   GLYGLUGLUTHRLYSALAMETGLUSERGLN
11   ARGALATYRARGPHEVALGLNGLYLYSASP
12   TRPGLYPHELYSLYSPHEILEARGARGASP
13   PHELEULEUASPGLUALAASNGLYLEULEU
14   PROASPASPLYSLEUTHRLEUPHECYSGLU
15   VALSERVALVALGLNASPSERVALASNILE
16   SERGLYGLNSERGLYPROSERSERGLY

Samples:

sample_1: MATH, [U-100% 13C; U-100% 15N], 0.5 mM; PUC 2.5 mM; sodium chloride 50.0 mM; potassium chloride 2.7 mM; sodium phosphate 10.0 mM; potassium phosphate 1.76 mM; DTT 5.0 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)CO; sample_1; sample_conditions_1sample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks