BMRB Entry 26635

Title:
Backbone 1H, and 15N Chemical Shift Assignments for human K-Ras4B-GDP
Deposition date:
2015-08-13
Original release date:
2015-09-11
Authors:
Abraham, Sherwin; Chavan, Tanmay; Banerjee, Avik
Citation:

Citation: Jang, Hyunbum; Banerjee, Avik; Chavan, Tanmay; Gaponenko, Vadim; Nussinov, Ruth. "Flexible-body motions of calmodulin and the farnesylated hypervariable region yield a high-affinity interaction enabling K-Ras4B membrane extraction"  J. Biol. Chem. 292, 12544-12559 (2017).
PubMed: 28623230

Assembly members:

Assembly members:
K-Ras4B, polymer, 188 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET42a

Data sets:
Data typeCount
15N chemical shifts171
1H chemical shifts171

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human K-Ras4B-GDP1

Entities:

Entity 1, human K-Ras4B-GDP 188 residues - Formula weight is not available

1   METTHRGLUTYRLYSLEUVALVALVALGLY
2   ALAGLYGLYVALGLYLYSSERALALEUTHR
3   ILEGLNLEUILEGLNASNHISPHEVALASP
4   GLUTYRASPPROTHRILEGLUASPSERTYR
5   ARGLYSGLNVALVALILEASPGLYGLUTHR
6   CYSLEULEUASPILELEUASPTHRALAGLY
7   GLNGLUGLUTYRSERALAMETARGASPGLN
8   TYRMETARGTHRGLYGLUGLYPHELEUCYS
9   VALPHEALAILEASNASNTHRLYSSERPHE
10   GLUASPILEHISHISTYRARGGLUGLNILE
11   LYSARGVALLYSASPSERGLUASPVALPRO
12   METVALLEUVALGLYASNLYSCYSASPLEU
13   PROSERARGTHRVALASPTHRLYSGLNALA
14   GLNASPLEUALAARGSERTYRGLYILEPRO
15   PHEILEGLUTHRSERALALYSTHRARGGLN
16   GLYVALASPASPALAPHETYRTHRLEUVAL
17   ARGGLUILEARGLYSHISLYSGLULYSMET
18   SERLYSASPGLYLYSLYSLYSLYSLYSLYS
19   SERLYSTHRLYSCYSVALILEMET

Samples:

sample_1: K-Ras4B, [U-13C; U-15N], mM; H2O 90 % v/v; D2O, [U-100% 2H], 10 % v/v; TRIS 50 mM; sodium chloride 50 mM; magenesium cloride 10 mM; beta-mercaptoethanol 5 mM

sample_conditions_1: ionic strength: 0.105 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks