BMRB Entry 26646

Title:
A Novel MHC-I Surface Targeted for Binding by the MCMV m06 Immunoevasin Revealed by Solution NMR
Deposition date:
2015-09-02
Original release date:
2016-07-15
Authors:
Sgourakis, Nikolaos; May, Nathan; Boyd, Lisa; Ying, Jinfa; Bax, Ad; Margulies, David
Citation:

Citation: Sgourakis, Nikolaos; May, Nathan; Boyd, Lisa; Ying, Jinfa; Bax, Ad; Margulies, David. "A Novel MHC-I Surface Targeted for Binding by the MCMV m06 Immunoevasin Revealed by Solution NMR"  J. Biol. Chem. 290, 28857-28868 (2015).
PubMed: 26463211

Assembly members:

Assembly members:
mini_H2-Ld, polymer, 180 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts506
15N chemical shifts170
1H chemical shifts170

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mini H2-Ld1

Entities:

Entity 1, mini H2-Ld 180 residues - Formula weight is not available

1   METGLYPROHISSERMETARGTYRTYRGLU
2   THRALATHRSERARGARGGLYLEUGLYGLU
3   PROARGTYRTHRSERVALGLYTYRVALASP
4   ASPLYSGLUPHEVALARGPHEASPSERASP
5   ALAGLUASNPROARGTYRGLUPROGLNVAL
6   PROTRPMETGLUGLNGLUGLYPROGLUTYR
7   TRPGLUARGILETHRGLNVALALALYSGLY
8   GLNGLUGLNTRPPHEARGVALASNLEUARG
9   THRLEULEUGLYTYRTYRASNGLNSERALA
10   GLYGLYTHRHISTHRLEUGLNARGMETTYR
11   GLYCYSASPVALGLYSERASPGLYARGLEU
12   LEUARGGLYTYRGLUGLNPHEALATYRASP
13   GLYCYSASPTYRILEALALEUASNGLUASP
14   LEUARGTHRTRPTHRALAALAASPMETALA
15   ALAGLNILETHRARGARGLYSTRPGLUGLN
16   ALAGLYALAALAGLUTYRTYRARGALATYR
17   LEUGLUGLYGLUCYSVALGLUTRPLEUHIS
18   ARGTYRLEULYSASNGLYASNALATHRLEU

Samples:

sample_1: mini H2-Ld, [U-100% 13C; U-100% 15N; U-80% 2H], 0.9 mM; H2O 90%; D2O 10%; PIPES 20 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 0.0257 M; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CA)CBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks