BMRB Entry 26687

Title:
Chemical shifts of calcium saturated CaM34 (CaM D93A, D129A) with the iNOS CaM-binding domain peptide
Deposition date:
2015-10-07
Original release date:
2016-02-12
Authors:
Piazza, Michael; Dieckmann, Thorsten
Citation:

Citation: Piazza, Michael; Guillemette, Guy; Dieckmann, Thorsten. "Chemical shift assignments of calmodulin constructs with EF hand mutations"  Biomol. NMR Assign. 10, 193-198 (2016).
PubMed: 26743203

Assembly members:

Assembly members:
CaM34, polymer, 148 residues, Formula weight is not available
iNOS, polymer, 17 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts419
15N chemical shifts144
1H chemical shifts882

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CaM341
2iNOS2

Entities:

Entity 1, CaM34 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEALALYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAALAILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, iNOS 17 residues - Formula weight is not available

1   LEULYSVALLEUVALLYSALAVALLEUPHE
2   ALACYSMETLEUMETARGLYS

Samples:

sample_1: CaM34, [U-99% 13C; U-99% 15N], 1 mM; potassium chloride 100 mM; iNOS 1 mM; sodium azide 0.2 mM; calcium chloride 1 mM

sample_conditions_1: ionic strength: 0.150 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks