BMRB Entry 26709

Title:
Backbone Assignment of Human linear diubiquitin
Deposition date:
2015-11-26
Original release date:
2016-01-14
Authors:
Halander, Jenny; Messias, Ana; Geerlof, Arie; Sattler, Michael
Citation:

Citation: Vincendeau, Michelle; Kamyar, Hadian; Messias, Ana; Brenke, Jara; Halander, Jenny; Griesbach, Richard; Greczmiel, Ute; Bertossi, Arianna; Stehle, Ralf; Nagel, Daniel; Demski, Kathrin; Velvarska, Hana; Niessing, Dierk; Geerlof, Arie; Sattler, Michael; Krappmann, Daniel. "Inhibition of Canonical NF-kB Signaling by a Small Molecule Targeting NEMO-Ubiquitin Interaction"  Sci. Rep. 6, 18934-18934 (2016).
PubMed: 26740240

Assembly members:

Assembly members:
linear_diubiquitin, polymer, 162 residues, 18220.8 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pASK-IBA-3plus

Data sets:
Data typeCount
13C chemical shifts255
15N chemical shifts132
1H chemical shifts132

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1linear diubiquitin1

Entities:

Entity 1, linear diubiquitin 162 residues - 18220.8 Da.

Residues 1-11 are non-native residues part of the N-terminal affinity tag.

1   METALASERHISHISHISHISHISHISGLY
2   ALAGLNILEPHEVALLYSTHRLEUTHRGLY
3   LYSTHRILETHRLEUGLUVALGLUPROSER
4   ASPTHRILEGLUASNVALLYSALALYSILE
5   GLNASPLYSGLUGLYILEPROPROASPGLN
6   GLNARGLEUILEPHEALAGLYLYSGLNLEU
7   GLUASPGLYARGTHRLEUSERASPTYRASN
8   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
9   LEUARGLEUARGGLYGLYMETGLNILEPHE
10   VALLYSTHRLEUTHRGLYLYSTHRILETHR
11   LEUGLUVALGLUPROSERASPTHRILEGLU
12   ASNVALLYSALALYSILEGLNASPLYSGLU
13   GLYILEPROPROASPGLNGLNARGLEUILE
14   PHEALAGLYLYSGLNLEUGLUASPGLYARG
15   THRLEUSERASPTYRASNILEGLNLYSGLU
16   SERTHRLEUHISLEUVALLEUARGLEUARG
17   GLYGLY

Samples:

sample_1: linear diubiquitin, [U-100% 13C; U-100% 15N], 0.9 mM; D2O 10%; Tris-HCl, [U-2H], 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 0.07 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AV III 800 MHz

Related Database Links:

UNIPROT P0CG47
PDB
AlphaFold Q9UPK7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks