BMRB Entry 26735

Title:
LdcI Assocating Domain of RavA Backbone Assignment
Deposition date:
2016-01-23
Original release date:
2016-06-08
Authors:
Sharpe, Simon; Houry, Walid A.; Chan, Sze Wah Samuel
Citation:

Citation: Chan, Sze Wah Samuel. "Mechanism of Amyloidogenesis of a Bacterial AAA+ Chaperone"  Structure 24, 1095-1109 (2016).
PubMed: 27265850

Assembly members:

Assembly members:
LARA3, polymer, 115 residues, 12609.5014 Da.

Natural source:

Natural source:   Common Name: Escherichia coli (strain K12)   Taxonomy ID: 511693   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p11

Data sets:
Data typeCount
13C chemical shifts359
15N chemical shifts111
1H chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LARA31

Entities:

Entity 1, LARA3 115 residues - 12609.5014 Da.

1   GLYHISMETGLNGLNSERASPLYSTHRALA
2   LEUTHRVALILEARGLEUGLYGLYILEPHE
3   SERARGARGGLNGLNTYRGLNLEUPROVAL
4   ASNVALTHRALASERTHRLEUTHRLEULEU
5   LEUGLNLYSPROLEULYSLEUHISASPMET
6   GLUVALVALHISILESERPHEGLUARGSER
7   ALALEUGLUGLNTRPLEUSERLYSGLYGLY
8   GLUILEARGGLYLYSLEUASNGLYILEGLY
9   PHEALAGLNLYSLEUASNLEUGLUVALASP
10   SERALAGLNHISLEUVALVALARGASPVAL
11   SERLEUGLNGLYSERTHRLEUALALEUPRO
12   GLYSERSERALAGLU

Samples:

LARA: LARA3, [[U-95% 13C; U-95% 15N]], 0.45 mM; H2O 90%; D2O 10%; potassium chloride 50 mM

Assignment: ionic strength: 50.000 mM; pH: 4.000; pressure: 1.000 atm; temperature: 280.150 K

Experiments:

NameSampleSample stateSample conditions
COCBCA (H[N[co[{CA|ca[C]}]]])LARAisotropicAssignment
CCCONH (C_ccoNH.relayed)LARAisotropicAssignment
3D HNCACBLARAisotropicAssignment
CACO (H[N[ca[CO]]])LARAisotropicAssignment
3D HNCALARAisotropicAssignment
3D HN(CO)CALARAisotropicAssignment
3D HNCOLARAisotropicAssignment
2D 1H-15N HSQC/HMQCLARAisotropicAssignment

Software:

CcpNmr_Analysis v2.3, CCPN - Aid

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks