BMRB Entry 26806

Title:
Chemical Shift Assignment of Tom1 VHS Domain
Deposition date:
2016-05-23
Original release date:
2016-10-13
Authors:
Capelluto, Daniel; Shanaiah, Narasimhamurthy; Ellena, Jeffrey
Citation:

Citation: Ellena, Jeffrey; Xiong, Wen; Zhao, Xiaolin; Shanaiah, Narasimhamurthy; Capelluto, Daniel. "Backbone 1H, 15N, and 13C resonance assignments of the Tom1 VHS domain"  Biomol. NMR Assign. 11, 1-4 (2017).
PubMed: 27704363

Assembly members:

Assembly members:
Tom1-VHS, polymer, 139 residues, 15563.88 Da.

Natural source:

Natural source:   Common Name: humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX4T3

Data sets:
Data typeCount
13C chemical shifts380
15N chemical shifts124
1H chemical shifts124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tom1-VHS1

Entities:

Entity 1, Tom1-VHS 139 residues - 15563.88 Da.

1   SERPROVALGLYGLNARGILEGLULYSALA
2   THRASPGLYSERLEUGLNSERGLUASPTRP
3   ALALEUASNMETGLUILECYSASPILEILE
4   ASNGLUTHRGLUGLUGLYPROLYSASPALA
5   LEUARGALAVALLYSLYSARGILEVALGLY
6   ASNLYSASNPHEHISGLUVALMETLEUALA
7   LEUTHRVALLEUGLUTHRCYSVALLYSASN
8   CYSGLYHISARGPHEHISVALLEUVALALA
9   SERGLNASPPHEVALGLUSERVALLEUVAL
10   ARGTHRILELEUPROLYSASNASNPROPRO
11   THRILEVALHISASPLYSVALLEUASNLEU
12   ILEGLNSERTRPALAASPALAPHEARGSER
13   SERPROASPLEUTHRGLYVALVALTHRILE
14   TYRGLUASPLEUARGARGLYSGLYLEU

Samples:

Tom1-VHS_sam: Tom1-VHS, [U-13C; U-15N], 800 uM; H2O 90%; D2O 10%

Tom1-VHS_con: pH: 7; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCTom1-VHS_samisotropicTom1-VHS_con
3D HNCACBTom1-VHS_samisotropicTom1-VHS_con
3D CBCA(CO)NHTom1-VHS_samisotropicTom1-VHS_con
3D HNCATom1-VHS_samisotropicTom1-VHS_con
3D HNCOTom1-VHS_samisotropicTom1-VHS_con

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking

Sparky, Goddard - processing

NMRview, Johnson, One Moon Scientific - processing

CCPNMR, Wayne Boucher and Tim Stevens - chemical shift assignment

NMR spectrometers:

  • Bruker Avance III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks