BMRB Entry 26810
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26810
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Marceau, Aimee; Felthousen, Jessica; Goetsch, Paul; Iness, Audra; Lee, Hsiau-Wei; Tripathi, Sarvind; Strome, Susan; Litovchick, Larisa; Rubin, Seth. "Structural basis for LIN54 recognition of CHR elements in cell cycle-regulated promoters" Nat. Comm. 7, 12301-12301 (2016).
PubMed: 27465258
Assembly members:
LIN54, polymer, 61 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEV
Entity Sequences (FASTA):
LIN54: GEFGESDRRHSKGCNCKRSG
CLKNYCECYEAKIMCSSICK
CIGCKNFEESPERKTLMHLA
D
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 176 |
| 15N chemical shifts | 62 |
| 1H chemical shifts | 65 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LIN54 | 1 |
| 2 | ZINC ION, 1 | 2 |
| 3 | ZINC ION, 2 | 2 |
| 4 | ZINC ION, 3 | 2 |
Entities:
Entity 1, LIN54 61 residues - Formula weight is not available
| 1 | GLY | GLU | PHE | GLY | GLU | SER | ASP | ARG | ARG | HIS | ||||
| 2 | SER | LYS | GLY | CYS | ASN | CYS | LYS | ARG | SER | GLY | ||||
| 3 | CYS | LEU | LYS | ASN | TYR | CYS | GLU | CYS | TYR | GLU | ||||
| 4 | ALA | LYS | ILE | MET | CYS | SER | SER | ILE | CYS | LYS | ||||
| 5 | CYS | ILE | GLY | CYS | LYS | ASN | PHE | GLU | GLU | SER | ||||
| 6 | PRO | GLU | ARG | LYS | THR | LEU | MET | HIS | LEU | ALA | ||||
| 7 | ASP |
Entity 2, ZINC ION, 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: LIN54, [U-100% 13C; U-100% 15N], 0.4 mM; MES 35 mM; sodium chloride 75 mM
sample_conditions_1: ionic strength: 75 mM; pH: 6; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment
NMRView, Johnson, One Moon Scientific - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks