BMRB Entry 26824

Title:
Lunasin assignment (reduced form)
Deposition date:
2016-06-21
Original release date:
2016-07-20
Authors:
Aleksis, Rihards; Jaudzems, Kristaps; Muceniece, Ruta; Liepinsh, Edvards
Citation:

Citation: Aleksis, Rihards; Jaudzems, Kristaps; Muceniece, Ruta; Liepinsh, Edvards. "Lunasin is a redox sensitive intrinsically disordered peptide with two transiently populated \u03b1-helical regions"  Peptides 85, 56-62 (2016).
PubMed: 27639324

Assembly members:

Assembly members:
Lunasin, polymer, 43 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDuet-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Lunasin: SKWQHQQDSCRKQLQGVNLT PCEKHIMEKIQGRGDDDDDD DDD

Data sets:
Data typeCount
13C chemical shifts249
15N chemical shifts101
1H chemical shifts570

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Lunasin1

Entities:

Entity 1, Lunasin 43 residues - Formula weight is not available

1   SERLYSTRPGLNHISGLNGLNASPSERCYS
2   ARGLYSGLNLEUGLNGLYVALASNLEUTHR
3   PROCYSGLULYSHISILEMETGLULYSILE
4   GLNGLYARGGLYASPASPASPASPASPASP
5   ASPASPASP

Samples:

Lunasin_sample: Lunasin, [U-99% 15N], 1 mM; D2O, [U-99% 2H], 7%; H2O 93%; sodium phosphate 20 mM; sodium azide 0.03%

pH35: ionic strength: 20 mM; pH: 3.5; pressure: 1 atm; temperature: 273 K

pH65: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCLunasin_sampleisotropicpH35
2D 1H-1H TOCSYLunasin_sampleisotropicpH35
2D 1H-1H NOESYLunasin_sampleisotropicpH35
3D 1H-15N NOESYLunasin_sampleisotropicpH35
2D 1H-13C HSQC aliphaticLunasin_sampleisotropicpH35
2D 1H-15N HSQCLunasin_sampleisotropicpH65
2D 1H-1H TOCSYLunasin_sampleisotropicpH65
2D 1H-1H NOESYLunasin_sampleisotropicpH65
3D 1H-15N NOESYLunasin_sampleisotropicpH65
2D 1H-13C HSQC aliphaticLunasin_sampleisotropicpH65

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks