BMRB Entry 26843

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for NCP15 nucleocapsid
Deposition date:
2016-07-04
Original release date:
2016-08-23
Authors:
LARUE, Valery; TISNE, Carine
Citation:

Citation: Larue, Valery; Catala, Marjorie; Belfetmi, Anissa; Zargarian, Loussine; Mauffret, Olivier; Tisne, Carine. "."  Biomol. NMR Assign. 12, 139-143 (2018).
PubMed: 29332151

Assembly members:

Assembly members:
HIV-1_NCP15_Nucleocapsid, polymer, 123 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: HIV   Taxonomy ID: 12721   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus HIV

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Data sets:
Data typeCount
13C chemical shifts296
15N chemical shifts104
1H chemical shifts283

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NCP151
2ZnCl22

Entities:

Entity 1, NCP15 123 residues - Formula weight is not available

1   ILEGLNLYSGLYASNPHEARGASNGLNARG
2   LYSTHRVALLYSCYSPHEASNCYSGLYLYS
3   GLUGLYHISILEALALYSASNCYSARGALA
4   PROARGLYSLYSGLYCYSTRPLYSCYSGLY
5   LYSGLUGLYHISGLNMETLYSASPCYSTHR
6   GLUARGGLNALAASNPHELEUGLYLYSILE
7   TRPPROSERHISLYSGLYARGPROGLYASN
8   PHELEUGLNSERARGPROGLUPROTHRALA
9   PROPROGLUGLUSERPHEARGPHEGLYGLU
10   GLUTHRTHRTHRPROSERGLNLYSGLNGLU
11   PROILEASPLYSGLULEUTYRPROLEUALA
12   SERLEUARGSERLEUPHEGLYSERASPPRO
13   SERSERGLN

Entity 2, ZnCl2 - 65.409 Da.

1   ZN

Samples:

sample_1: NCP15 Nucleocapsid, [U-100% 13C; U-100% 15N], 0.8 mM; acetate buffer 25 mM; NaCl 25 mM; ZnCl2 0.1 mM; Mercaptoethanol 0.1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 25 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 950 MHz
  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks