BMRB Entry 26893

Title:
Backbone resonance assignments of monomeric SOD1 in dilute environment
Deposition date:
2016-09-13
Original release date:
2016-10-14
Authors:
Iwakawa, Naoto; Morimoto, Daichi
Citation:

Citation: Iwakawa, Naoto; Morimoto, Daichi; Walinda, Erik; Sugase, Kenji; Shirakawa, Masahiro. "Backbone resonance assignments of monomeric SOD1 in dilute and crowded environments"  Biomol. NMR Assign. 11, 81-84 (2017).
PubMed: 28025808

Assembly members:

Assembly members:
monomeric_SOD1_H46W, polymer, 110 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3a-d

Data sets:
Data typeCount
13C chemical shifts310
15N chemical shifts105
1H chemical shifts105

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SOD11

Entities:

Entity 1, SOD1 110 residues - Formula weight is not available

1   ALATHRLYSALAVALALAVALLEULYSGLY
2   ASPGLYPROVALGLNGLYILEILEASNPHE
3   GLUGLNLYSGLUSERASNGLYPROVALLYS
4   VALTRPGLYSERILELYSGLYLEUTHRGLU
5   GLYLEUHISGLYPHETRPVALHISGLYALA
6   GLYGLYASPLEUGLYASNVALTHRALAASP
7   LYSASPGLYVALALAASPVALSERILEGLU
8   ASPSERVALILESERLEUSERGLYASPHIS
9   SERILEILEGLYARGTHRLEUVALVALHIS
10   GLULYSALAGLYALAGLYALAGLYSERARG
11   LEUALASERGLYVALILEGLYILEALAGLN

Samples:

sample_1: monomeric SOD1 H46W, [U-100% 13C; U-100% 15N], 1.0 mM; Bis-Tris 10 mM

sample_conditions_1: pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks