BMRB Entry 26915

Name: Backbone Chemical Shift of Spy (Spheroplast protein Y)
Published: 2017-05-23

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26915

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Chemical Shift of Spy (Spheroplast protein Y)

Deposition date:

2016-10-06

Original release date:

2017-05-23

Authors:

He, Lichun; Hiller, Sebastian

Citation:

Citation: He, Lichun; Sharpe, Timothy; Mazur, Adam; Hiller, Sebastian. "A molecular mechanism of chaperone-client recognition" Sci. Adv. 2, e1601625-e1601625 (2016).
PubMed: 28138538

Assembly members:

Assembly members:
Spy, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Spy: ADTTTAAPADAKPMMHHKGK FGPHQDMMFKDLNLTDAQKQ QIREIMKGQRDQMKRPPLEE RRAMHDIITSDTFDKVKAEA QIAKMEEQRKANMLAHMETQ NKIYNILTPEQKKQFNANFE KRLTERPAAKGKMPATAE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	366
15N chemical shifts	126
1H chemical shifts	126

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Spy	1

Entities:

Entity 1, Spy 138 residues - Formula weight is not available

1

ALA

ASP

THR

ALA

PRO

ALA

ASP

2

ALA

LYS

PRO

MET

HIS

LYS

GLY

LYS

3

PHE

GLY

PRO

HIS

GLN

ASP

MET

PHE

LYS

4

ASP

LEU

ASN

LEU

THR

ASP

ALA

GLN

LYS

GLN

5

GLN

ILE

ARG

GLU

ILE

MET

LYS

GLY

GLN

ARG

6

ASP

GLN

MET

LYS

ARG

PRO

LEU

GLU

7

ARG

ALA

MET

HIS

ASP

ILE

THR

SER

8

ASP

THR

PHE

ASP

LYS

VAL

LYS

ALA

GLU

ALA

9

GLN

ILE

ALA

LYS

MET

GLU

GLN

ARG

LYS

10

ALA

ASN

MET

LEU

ALA

HIS

MET

GLU

THR

GLN

11

ASN

LYS

ILE

TYR

ASN

ILE

LEU

THR

PRO

GLU

12

GLN

LYS

GLN

PHE

ASN

ALA

ASN

PHE

GLU

13

LYS

ARG

LEU

THR

GLU

ARG

PRO

ALA

LYS

14

GLY

LYS

MET

PRO

ALA

THR

ALA

GLU

Samples:

sample_1: Spy, [U-99% 13C; U-99% 15N], 1 mM; NaP buffer 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks