BMRB Entry 26920

Title:
1H, 13C and 15N backbone resonance assignment of the TPR2B domain of mouse STI1
Deposition date:
2016-10-19
Original release date:
2017-08-11
Authors:
Choy, Wing-Yiu; Maciejewski, Andrzej; Prado, Marco
Citation:

Citation: Maciejewski, Andrzej; Prado, Vania; Prado, Marco; Choy, Wing-Yiu. "Molecular basis for the interaction between stress-inducible phosphoprotein 1 (STIP1) and S100A1"  Biochem. J. 474, 1853-1866 (2017).
PubMed: 28408431

Assembly members:

Assembly members:
TPR2B, polymer, 137 residues, 15795.8 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDEST17

Data sets:
Data typeCount
13C chemical shifts247
15N chemical shifts122
1H chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1TPR2B1

Entities:

Entity 1, TPR2B 137 residues - 15795.8 Da.

N-terminal glycine is a non-native residue from a TEV recognition site

1   GLYGLNGLUARGLEUALATYRILEASNPRO
2   ASPLEUALALEUGLUGLULYSASNLYSGLY
3   ASNGLUCYSPHEGLNLYSGLYASPTYRPRO
4   GLNALAMETLYSHISTYRTHRGLUALAILE
5   LYSARGASNPROARGASPALALYSLEUTYR
6   SERASNARGALAALACYSTYRTHRLYSLEU
7   LEUGLUPHEGLNLEUALALEULYSASPCYS
8   GLUGLUCYSILEGLNLEUGLUPROTHRPHE
9   ILELYSGLYTYRTHRARGLYSALAALAALA
10   LEUGLUALAMETLYSASPTYRTHRLYSALA
11   METASPVALTYRGLNLYSALALEUASPLEU
12   ASPSERSERCYSLYSGLUALAALAASPGLY
13   TYRGLNARGCYSMETMETALAGLNTYRASN
14   ARGHISASPSERPROGLUASP

Samples:

sample_1: TPR2B, [U-99% 13C; U-99% 15N], 500 uM; sodium phosphate 50 mM; sodium chloride 50 mM; DTT 1 mM; DSS 100 uM

sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks