BMRB Entry 26923

Title:
Backbone and Ile, Leu, Val Methyl Chemical Shift Assignments for Rhizopus microsporus var. chinensis lipase
Deposition date:
2016-10-24
Original release date:
2018-02-09
Authors:
Zhang, Meng; Yu, Xiao-Wei; G.V.T., Swapna; Liu, Gaohua; Xiao, Rong; Xu, Yan; Montelione, Gaetano
Citation:

Citation: Zhang, Meng; Yu, Xiao-Wei; G.V.T., Swapna; Liu, Gaohua; Xiao, Rong; Xu, Yan; Montelione, Gaetano. "Backbone and Ile-delta1, Leu, Val methyl 1H, 15N, and 13C, chemical shift assignments for Rhizopus chinensis lipase"  Biomol. NMR Assign. 12, 63-68 (2018).
PubMed: 28929427

Assembly members:

Assembly members:
RCL, polymer, 296 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rhizopus microsporus   Taxonomy ID: 58291   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Rhizopus microsporus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: MBP3

Data sets:
Data typeCount
13C chemical shifts554
15N chemical shifts228
1H chemical shifts477

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RCL1

Entities:

Entity 1, RCL 296 residues - Formula weight is not available

1   ASPTHRGLUTHRVALGLYGLYMETTHRLEU
2   ASPLEUPROGLUASNPROPROPROILEPRO
3   ALATHRSERTHRALAPROSERSERASPSER
4   GLYGLUVALVALTHRALATHRALAALAGLN
5   ILELYSGLULEUTHRASNTYRALAGLYVAL
6   ALAALATHRALATYRCYSARGSERVALVAL
7   PROGLYTHRLYSTRPASPCYSLYSGLNCYS
8   LEULYSTYRVALPROASPGLYLYSLEUILE
9   LYSTHRPHETHRSERLEULEUTHRASPTHR
10   ASNGLYPHEILELEUARGSERASPALAGLN
11   LYSTHRILETYRVALTHRPHEARGGLYTHR
12   ASNSERPHEARGSERALAILETHRASPMET
13   VALPHETHRPHETHRASPTYRSERPROVAL
14   LYSGLYALALYSVALHISALAGLYPHELEU
15   SERSERTYRASNGLNVALVALLYSASPTYR
16   PHEPROVALVALGLNASPGLNLEUTHRALA
17   TYRPROASPTYRLYSVALILEVALTHRGLY
18   HISSERLEUGLYGLYALAGLNALALEULEU
19   ALAGLYMETASPLEUTYRGLNARGGLULYS
20   ARGLEUSERPROLYSASNLEUSERILETYR
21   THRVALGLYCYSPROARGVALGLYASNASN
22   ALAPHEALATYRTYRVALASPSERTHRGLY
23   ILEPROPHEHISARGTHRVALHISLYSARG
24   ASPILEVALPROHISVALPROPROGLNALA
25   PHEGLYTYRLEUHISPROGLYVALGLUSER
26   TRPILELYSGLUASPPROALAASPVALGLN
27   ILECYSTHRSERASNILEGLUTHRLYSGLN
28   CYSSERASNSERILEVALPROPHETHRSER
29   ILEALAASPHISLEUTHRTYRPHEGLYILE
30   ASNGLUGLYSERCYSLEU

Samples:

sample_1: RCL, [U-13C; U-15N; U-2H], 0.12 mM; DMSO, [U-99% 2H], 2%; DSS 50 uM; D2O, [U-99% 2H], 10%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - peak picking

TOPSPIN, Bruker Biospin - collection

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

GB EF405962.2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks