BMRB Entry 26942

Name: Backbone and Side-Chain (1)H, (13)C, and (15)N Chemical Shift Assignments for the homodimeric Histone-like DNA binding protein (Hup) of Helicobacter pylori
Published: 2018-03-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26942

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and Side-Chain (1)H, (13)C, and (15)N Chemical Shift Assignments for the homodimeric Histone-like DNA binding protein (Hup) of Helicobacter pylori

Deposition date:

2016-11-12

Original release date:

2018-03-16

Authors:

Jaiswal, Nancy; Raikwal, Nisha; Pandey, Himanshu; Arora, Ashish; Kumar, Dinesh

Citation:

Citation: Jaiswal, Nancy; Raikwal, Nisha; Pandey, Himanshu; Agarwal, Nipanshu; Arora, Ashish; Poluri, Krishna Mohan; Kumar, Dinesh. "NMR elucidation of monomer-dimer transition and conformational heterogeneity in histone-like DNA binding protein of Helicobacter pylori" Magn. Reson. Chem. 56, 285-299 (2018).
PubMed: 29241299

Assembly members:

Assembly members:
Hup, polymer, 98 residues, 10772 Da.

Natural source:

Natural source: Common Name: Helicobacter pylori Taxonomy ID: 210 Superkingdom: Bacteria Kingdom: not available Genus/species: Helicobacter pylori

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE80L

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hup: GAMEMNKAEFIDLVKEAGKY NSKREAEEAISAFTLAVETA LSKGESVELIGFGKFETAEQ KGKEGKVPGSDKTYKTEDKR VPKFKPGKTLKQKVEEGK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	570
15N chemical shifts	170
1H chemical shifts	421

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Hup, Monomer 1	1
2	Hup, Monomer 2	1

Entities:

Entity 1, Hup, Monomer 1 98 residues - 10772 Da.

Residue -4 to -1 represent cloning artifacts

1

GLY

ALA

MET

GLU

MET

ASN

LYS

ALA

GLU

PHE

2

ILE

ASP

LEU

VAL

LYS

GLU

ALA

GLY

LYS

TYR

3

ASN

SER

LYS

ARG

GLU

ALA

GLU

ALA

ILE

4

SER

ALA

PHE

THR

LEU

ALA

VAL

GLU

THR

ALA

5

LEU

SER

LYS

GLY

GLU

SER

VAL

GLU

LEU

ILE

6

GLY

PHE

GLY

LYS

PHE

GLU

THR

ALA

GLU

GLN

7

LYS

GLY

LYS

GLU

GLY

LYS

VAL

PRO

GLY

SER

8

ASP

LYS

THR

TYR

LYS

THR

GLU

ASP

LYS

ARG

9

VAL

PRO

LYS

PHE

LYS

PRO

GLY

LYS

THR

LEU

10

LYS

GLN

LYS

VAL

GLU

GLY

LYS

Samples:

sample_1: Hup, [U-99% 13C; U-99% 15N], 1 mM; D2O, [U-100% 2H], 10%; H2O 90%; sodium azide 0.1%; sodium chloride 300 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 0.079 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0.079 M; pH: 6.0; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
HNN	sample_1	isotropic	sample_conditions_1
hNCAnH	sample_1	isotropic	sample_conditions_1
HN(CA)CO	sample_1	isotropic	sample_conditions_1
IntraHNCA	sample_1	isotropic	sample_conditions_1
NNH-NOESY	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.9.1, Freely Available - chemical shift assignment, data analysis, structure solution

NMR spectrometers:

Bruker Avance 800 MHz