BMRB Entry 26963

Title:
PSEUDOMONAS AERUGINOSA ICP
Deposition date:
2016-11-30
Original release date:
2016-12-02
Authors:
Fu, Yinan; Smith, Brian
Citation:

Citation: Fu, Yinan. "Structure and Dynamics of Pseudomonas Aeruginosa Icp"  Thesis University of Glasgow .

Assembly members:

Assembly members:
paicphis, polymer, 132 residues, 14563.1313 Da.

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data typeCount
13C chemical shifts380
15N chemical shifts105
1H chemical shifts761

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1paicphis1

Entities:

Entity 1, paicphis 132 residues - 14563.1313 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METGLNLYSPROVALVALTHRLEUASPASP
4   ALAASPASPCYSSERPROLEULYSLEUTHR
5   GLNGLYGLNGLULEUVALLEUTHRLEUPRO
6   SERASNPROTHRTHRGLYPHEARGTRPGLU
7   LEUARGASNPROALAALASERVALLEULYS
8   ARGLEUGLYPROGLUVALTYRSERASNSER
9   GLUGLUASPSERGLYLEUVALGLYSERGLY
10   GLYGLUSERTHRTRPARGPHEARGVALALA
11   ALASERGLYASPASPARGLEUGLULEUVAL
12   TYRARGARGPROTRPGLULYSASPALAGLU
13   PROALAGLUSERPHESERCYSALAILEGLN
14   VALARG

Samples:

sample_1: PA-ICP, [U-100% 13C; U-100% 15N], 1 mM; Na2HPO4 25 mM; NaCl 75 mM; azide 0.01%

sample_conditions_1: ionic strength: 0.125 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
hbcbcgcdcehe (H[C[caro]])sample_1isotropicsample_conditions_1
hbcbcgcdhd (H[C[caro]])sample_1isotropicsample_conditions_1
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC/HMQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
newCBCACONH (H[N[co[{CA|ca[C]}]]])sample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC/HMQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC/HMQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC/HMQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC/HMQCsample_1isotropicsample_conditions_1

Software:

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

CcpNmr_Analysis v2.4, CCPN - data analysis

azara vany, Boucher - processing

topspin vany, Bruker Biospin - collection

CCPNMR_Analysis v1.0, CCPN - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks