BMRB Entry 26975

Title:
AILV Methyl Resonance Assignments for G protein alpha i3 subunit in the GDP-bound state
Deposition date:
2016-12-17
Original release date:
2017-02-21
Authors:
Toyama, Yuki; Kano, Hanaho; Mase, Yoko; Yokogawa, Mariko; Osawa, Masanori; Shimada, Ichio
Citation:

Citation: Toyama, Yuki; Kano, Hanaho; Mase, Yoko; Yokogawa, Mariko; Osawa, Masanori; Shimada, Ichio. "Dynamic regulation of GDP binding to G proteins revealed by magnetic field dependent NMR relaxation analyses"  Nat. Commun. 8, .-. (2017).
PubMed: 28223697

Assembly members:

Assembly members:
G_protein_alpha_subunit_i3, polymer, 359 residues, Formula weight is not available
entity_GDP, non-polymer, 443.201 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24d(+)

Data sets:
Data typeCount
13C chemical shifts96
1H chemical shifts288

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1G alphai 31
2GDP2

Entities:

Entity 1, G alphai 3 359 residues - Formula weight is not available

1   GLYPROASPASPHISMETGLYCYSTHRLEU
2   SERALAGLUASPLYSALAALAVALGLUARG
3   SERLYSMETILEASPARGASNLEUARGGLU
4   ASPGLYGLULYSALAALALYSGLUVALLYS
5   LEULEULEULEUGLYALAGLYGLUSERGLY
6   LYSSERTHRILEVALLYSGLNMETLYSILE
7   ILEHISGLUASPGLYTYRSERGLUASPGLU
8   CYSLYSGLNTYRLYSVALVALVALTYRSER
9   ASNTHRILEGLNSERILEILEALAILEILE
10   ARGALAMETGLYARGLEULYSILEASPPHE
11   GLYGLUALAALAARGALAASPASPALAARG
12   GLNLEUPHEVALLEUALAGLYSERALAGLU
13   GLUGLYVALMETTHRPROGLULEUALAGLY
14   VALILELYSARGLEUTRPARGASPGLYGLY
15   VALGLNALACYSPHESERARGSERARGGLU
16   TYRGLNLEUASNASPSERALASERTYRTYR
17   LEUASNASPLEUASPARGILESERGLNSER
18   ASNTYRILEPROTHRGLNGLNASPVALLEU
19   ARGTHRARGVALLYSTHRTHRGLYILEVAL
20   GLUTHRHISPHETHRPHELYSASPLEUTYR
21   PHELYSMETPHEASPVALGLYGLYGLNARG
22   SERGLUARGLYSLYSTRPILEHISCYSPHE
23   GLUGLYVALTHRALAILEILEPHECYSVAL
24   ALALEUSERASPTYRASPLEUVALLEUALA
25   GLUASPGLUGLUMETASNARGMETHISGLU
26   SERMETLYSLEUPHEASPSERILECYSASN
27   ASNLYSTRPPHETHRGLUTHRSERILEILE
28   LEUPHELEUASNLYSLYSASPLEUPHEGLU
29   GLULYSILELYSARGSERPROLEUTHRILE
30   CYSTYRPROGLUTYRTHRGLYSERASNTHR
31   TYRGLUGLUALAALAALATYRILEGLNCYS
32   GLNPHEGLUASPLEUASNARGARGLYSASP
33   THRLYSGLUILETYRTHRHISPHETHRCYS
34   ALATHRASPTHRLYSASNVALGLNPHEVAL
35   PHEASPALAVALTHRASPVALILEILELYS
36   ASNASNLEULYSGLUCYSGLYLEUTYR

Entity 2, GDP - C10 H15 N5 O11 P2 - 443.201 Da.

1   GDP

Samples:

sample_1: G protein alpha subunit i3, [U-2H, AILVproS 1H,13C], 800 uM; HEPES 20 mM; DTT 5 mM; GUANOSINE-5'-DIPHOSPHATE 500 uM; DSS 100 uM; D2O 100%

sample_conditions_1: ionic strength: 20 mM; pD: 7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1

Software:

TOPSPIN vv2.1, Bruker Biospin - collection, processing

SPARKY v3.114, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

UNP P08754
GB AAM12621.1
AlphaFold Q5TZX1