BMRB Entry 26998

Name: Rabbit Prp 90-231 NH assignments
Published: 2017-04-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26998

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Rabbit Prp 90-231 NH assignments

Deposition date:

2017-01-18

Original release date:

2017-04-26

Authors:

Hansen, D. Fleming; Rizk, Paul; Chong, P. Andrew; Forman-Kay, Julie

Citation:

Citation: Malevanets, Anatoly; Chong, P. Andrew; Hansen, D. Fleming; Rizk, Paul; Sun, Yulong; Lin, Hong; Muhandiram, Ranjith; Chakrabartty, Avi; Kay, Lewis; Forman-Kay, Julie; Wodak, Shoshana. "Interplay of buried histidine protonation and protein stability in prion misfolding" Sci. Rep. 7, 882-882 (2017).
PubMed: 28408762

Assembly members:

Assembly members:
Rabbit_PrP_90-231, polymer, 144 residues, Formula weight is not available

Natural source:

Natural source: Common Name: rabbit Taxonomy ID: 9986 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oryctolagus cuniculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pBS152v

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rabbit_PrP_90-231: GAMGGQGGTHNQWGKPSKPK TSMKHVAGAAAAGAVVGGLG GYMLGSAMSRPLIHFGNDYE DRYYRENMYRYPNQVYYRPV DQYSNQNSFVHDCVNITVKQ HTVTTTTKGENFTETDIKIM ERVVEQMCITQYQQESQAAY QRAA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
15N chemical shifts	85
1H chemical shifts	85

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Rabbit Prp 90-231	1

Entities:

Entity 1, Rabbit Prp 90-231 144 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

GLN

GLY

THR

HIS

2

ASN

GLN

TRP

GLY

LYS

PRO

SER

LYS

PRO

LYS

3

THR

SER

MET

LYS

HIS

VAL

ALA

GLY

ALA

4

ALA

GLY

ALA

VAL

GLY

LEU

GLY

5

GLY

TYR

MET

LEU

GLY

SER

ALA

MET

SER

ARG

6

PRO

LEU

ILE

HIS

PHE

GLY

ASN

ASP

TYR

GLU

7

ASP

ARG

TYR

ARG

GLU

ASN

MET

TYR

ARG

8

TYR

PRO

ASN

GLN

VAL

TYR

ARG

PRO

VAL

9

ASP

GLN

TYR

SER

ASN

GLN

ASN

SER

PHE

VAL

10

HIS

ASP

CYS

VAL

ASN

ILE

THR

VAL

LYS

GLN

11

HIS

THR

VAL

THR

LYS

GLY

GLU

12

ASN

PHE

THR

GLU

THR

ASP

ILE

LYS

ILE

MET

13

GLU

ARG

VAL

GLU

GLN

MET

CYS

ILE

THR

14

GLN

TYR

GLN

GLU

SER

GLN

ALA

TYR

15

GLN

ARG

ALA

Samples:

sample_1: Rabbit PrP 90-231, [U-99% 13C; U-99% 15N], 0.5 mM; sodium acetate 20 mM

sample_2: Rabbit PrP 90-231, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 20 mM; pH: 5.2; pressure: 1 atm; temperature: 293 K

sample_conditions_2: ionic strength: 20 mM; pH: 7; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_2
3D HNCACB	sample_2	isotropic	sample_conditions_2
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks