BMRB Entry 27046

Name: Backbone chemical shift assignments for the oxidized alpha-crystallin domain of HSP27 (HSPB1)
Published: 2017-06-30

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27046

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shift assignments for the oxidized alpha-crystallin domain of HSP27 (HSPB1)

Deposition date:

2017-03-09

Original release date:

2017-06-30

Authors:

Alderson, T.; Gastall, Heidi; Benesch, Justin; Baldwin, Andrew

Citation:

Citation: Alderson, T Reid; Benesch, Justin; Baldwin, Andrew. "Proline isomerization in the C-terminal region of HSP27" Cell Stress Chaperones 22, 639-651 (2017).
PubMed: 28547731

Assembly members:

Assembly members:
cHSP27, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cHSP27: GVSEIRHTADRWRVSLDVNH FAPDELTVKTKDGVVEITGK HEERQDEHGYISRCFTRKYT LPPGVDPTQVSSSLSPEGTL TVEAPMPK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	249
15N chemical shifts	77
1H chemical shifts	82

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	cHSP27_dimer	1
2	cHSP27_dimer2	1

Entities:

Entity 1, cHSP27_dimer 88 residues - Formula weight is not available

1

GLY

VAL

SER

GLU

ILE

ARG

HIS

THR

ALA

ASP

2

ARG

TRP

ARG

VAL

SER

LEU

ASP

VAL

ASN

HIS

3

PHE

ALA

PRO

ASP

GLU

LEU

THR

VAL

LYS

THR

4

LYS

ASP

GLY

VAL

GLU

ILE

THR

GLY

LYS

5

HIS

GLU

ARG

GLN

ASP

GLU

HIS

GLY

TYR

6

ILE

SER

ARG

CYS

PHE

THR

ARG

LYS

TYR

THR

7

LEU

PRO

GLY

VAL

ASP

PRO

THR

GLN

VAL

8

SER

LEU

SER

PRO

GLU

GLY

THR

LEU

9

THR

VAL

GLU

ALA

PRO

MET

PRO

LYS

Samples:

sample_1: cHSP27, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 30 mM; EDTA 2 mM

sample_conditions_1: ionic strength: 30 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks