BMRB Entry 27053

Name: NMR chemical shift assignments of a 22mer G-quadruplex formed within the KRAS proto-oncogene promoter region
Published: 2017-03-31

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27053

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR chemical shift assignments of a 22mer G-quadruplex formed within the KRAS proto-oncogene promoter region

Deposition date:

2017-03-23

Original release date:

2017-03-31

Authors:

Salgado, Gilmar; Marquevielle, Julien; Kerkour, Abdelaziz; Mergny, Jean-Louis

Citation:

Citation: Kerkour, Abdelaziz; Marquevielle, Julien; Ivashchenko, Stefaniia; Yatsunyk, Liliya; Mergny, Jean-Louis; Salgado, Gilmar. "High-resolution 3D NMR structure of the KRAS proto-oncogene promoter reveals key features of a G-quadruplex involved in transcriptional regulation" J. Biol. Chem. 292, 8082-8091 (2017).
PubMed: 28330874

Assembly members:

Assembly members:
KRAS22RT, polymer, 22 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KRAS22RT: AGGGCGGTGTGGGAATAGGG AA

Data sets:

assigned_chemical_shifts
- KRAS22RT_chemicalshifts
spectral_peak_list
- KRAS22RT_293K_list

Data type	Count
1H chemical shifts	218

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	G4 within promoter region	1

Entities:

Entity 1, G4 within promoter region 22 residues - Formula weight is not available

1

DA

DG

DC

DG

DT

DG

DT

2

DG

DA

DT

DA

DG

3

DA

Samples:

sample_KRAS22RT: KRAS22RT 2.5 mM; KCl 90 mM; KPi 20 mM

sample_conditions_1: ionic strength: 110 mM; pH: 6.65; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_KRAS22RT	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz