BMRB Entry 27064

Name: Solution structure of the IgI domain of CD147
Published: 2017-12-14

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27064

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the IgI domain of CD147

Deposition date:

2017-04-05

Original release date:

2017-12-14

Authors:

Jin, Shujuan; Xia, Bin

Citation:

Citation: Jin, Shujuan; Ding, Pengfei; Chu, Pengxiang; Li, Hongwei; Sun, Jianbo; Liang, Dehai; Song, Fei; Xia, Bin. "Zn(II) can mediate self-association of the extracellular C-terminal domain of CD147." Protein Cell 9, 310-315 (2018).
PubMed: 28822092

Assembly members:

Assembly members:
CD147, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CD147: IQLHGPPRVKAVKSSEHINE GETAMLVCKSESVPPVTDWA WYKITDSEDKALMNGSESRF FVSSSQGRSELHIENLNMEA DPGQYRCNGTSSKGSDQAII TLRVRSH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	430
15N chemical shifts	113
1H chemical shifts	698

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CD147	1

Entities:

Entity 1, CD147 107 residues - Formula weight is not available

1

ILE

GLN

LEU

HIS

GLY

PRO

ARG

VAL

LYS

2

ALA

VAL

LYS

SER

GLU

HIS

ILE

ASN

GLU

3

GLY

GLU

THR

ALA

MET

LEU

VAL

CYS

LYS

SER

4

GLU

SER

VAL

PRO

VAL

THR

ASP

TRP

ALA

5

TRP

TYR

LYS

ILE

THR

ASP

SER

GLU

ASP

LYS

6

ALA

LEU

MET

ASN

GLY

SER

GLU

SER

ARG

PHE

7

PHE

VAL

SER

GLN

GLY

ARG

SER

GLU

8

LEU

HIS

ILE

GLU

ASN

LEU

ASN

MET

GLU

ALA

9

ASP

PRO

GLY

GLN

TYR

ARG

CYS

ASN

GLY

THR

10

SER

LYS

GLY

SER

ASP

GLN

ALA

ILE

11

THR

LEU

ARG

VAL

ARG

SER

HIS

Samples:

sample_1: CD147, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 50 mM; sodium chloride 50 mM; EDTA 5 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks