BMRB Entry 27106

Title:
Human BRM AT-hook and Bromodomain
Deposition date:
2017-05-15
Original release date:
2017-06-01
Authors:
Morrison, Emma
Citation:

Citation: Morrison, Emma; Sanchez, Julio; Ronan, Jehnna; Farrell, Daniel; Varzavand, Katayoun; Johnson, Jenna; Gu, Brian; Crabtree, Gerald; Musselman, Catherine. "DNA binding drives the association of BRG1/hBRM bromodomains with nucleosomes"  Nat Commun. 8, 16080-16080 (2017).
PubMed: 28706277

Assembly members:

Assembly members:
BRM_AT-BRD, polymer, 153 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDEST15

Data sets:
Data typeCount
13C chemical shifts190
15N chemical shifts131
1H chemical shifts131

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AT-BRD1

Entities:

Entity 1, AT-BRD 153 residues - Formula weight is not available

1   GLYPROLYSGLUASPVALGLULYSALALYS
2   LYSARGARGGLYARGPROPROALAGLULYS
3   LEUSERPROASNPROPROLYSLEUTHRLYS
4   GLNMETASNALAILEILEASPTHRVALILE
5   ASNTYRLYSASPARGCYSASNVALGLULYS
6   VALPROSERASNSERGLNLEUGLUILEGLU
7   GLYASNSERSERGLYARGGLNLEUSERGLU
8   VALPHEILEGLNLEUPROSERARGLYSGLU
9   LEUPROGLUTYRTYRGLULEUILEARGLYS
10   PROVALASPPHELYSLYSILELYSGLUARG
11   ILEARGASNHISLYSTYRARGSERLEUGLY
12   ASPLEUGLULYSASPVALMETLEULEUCYS
13   HISASNALAGLNTHRPHEASNLEUGLUGLY
14   SERGLNILETYRGLUASPSERILEVALLEU
15   GLNSERVALPHELYSSERALAARGGLNLYS
16   ILEALALYS

Samples:

sample_1: BRM, [U-100% 13C; U-100% 15N], 0.5 mM; KPi 50 mM; KCl 50 mM

sample_conditions_1: ionic strength: 129 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
HNCAsample_1isotropicsample_conditions_1

Software:

CCPNMR, CCPN - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks