BMRB Entry 27127

Title:
1H, 13C, 15N Chemical Shift Assignments for vaccinia topoisomerase 1B N terminal domain (1-80)
Deposition date:
2017-06-06
Original release date:
2017-06-19
Authors:
Reed, Benjamin
Citation:

Citation: Reed, Benjamin; Yakovleva, Lyudmila; Shuman, Stewart; Ghose, Ranajeet. "Characterization of DNA Binding by the Isolated N-Terminal Domain of Vaccinia Virus DNA Topoisomerase IB"  Biochemistry 56, 3307-3317 (2017).
PubMed: 28570045

Assembly members:

Assembly members:
TopN, polymer, 82 residues, 9596.8 Da.

Natural source:

Natural source:   Common Name: Vaccinia virus   Taxonomy ID: 10254   Superkingdom: Poxviridae   Kingdom: Chordopoxvirinae   Genus/species: Orthopoxvirus vaccinia

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet15B

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts315
15N chemical shifts77
1H chemical shifts77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TopN monomer1

Entities:

Entity 1, TopN monomer 82 residues - 9596.8 Da.

1   GLYSERMETARGALALEUPHETYRLYSASP
2   GLYLYSLEUPHETHRASPASNASNPHELEU
3   ASNPROVALSERASPASPASNPROALATYR
4   GLUVALLEUGLNHISVALLYSILEPROTHR
5   HISLEUTHRASPVALVALVALTYRGLUGLN
6   THRTRPGLUGLUALALEUTHRARGLEUILE
7   PHEVALGLYSERASPSERLYSGLYARGARG
8   GLNTYRPHETYRGLYLYSMETHISVALGLN
9   ASNARG

Samples:

sample_1: D2O, [U-2H], 10%; H2O 90%; sodium phosphate 50 mM; sodium chloride 150 mM; TopN, [U-100% 13C; U-100% 15N], 300 uM

sample_conditions_1: ionic strength: 0.35 M; pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D CCcoCNH TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

NMRView v9.2.0-b, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks