BMRB Entry 27137

Title:
HypF-N backbone assignment in toxic oligomer solution condition
Deposition date:
2017-06-12
Original release date:
2018-06-21
Authors:
Patel, Jayneil; De Simone, Alfonso; Xu, Yingqi
Citation:

Citation: Patel, Jayneil; Xu, Yingqi; Capitini, Claudia; Chiti, Fabrizio; De Simone, Alfonso. "Backbone NMR assignments of HypF-N under conditions generating toxic and non-toxic oligomers"  Biomol. NMR Assign. 12, 273-277 (2018).
PubMed: 29786756

Assembly members:

Assembly members:
N_terminal_domain_of_HypF_(HypF-N), polymer, 92 residues, 10464.89 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE_Th

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts236
15N chemical shifts77
1H chemical shifts148

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HypF-N1

Entities:

Entity 1, HypF-N 92 residues - 10464.89 Da.

1   GLYSERALALYSASNTHRSERCYSGLYVAL
2   GLNLEUARGILEARGGLYLYSVALGLNGLY
3   VALGLYPHEARGPROPHEVALTRPGLNLEU
4   ALAGLNGLNLEUASNLEUHISGLYASPVAL
5   CYSASNASPGLYASPGLYVALGLUVALARG
6   LEUARGGLUASPPROGLUTHRPHELEUVAL
7   GLNLEUTYRGLNHISCYSPROPROLEUALA
8   ARGILEASPSERVALGLUARGGLUPROPHE
9   ILETRPSERGLNLEUPROTHRGLUPHETHR
10   ILEARG

Samples:

HypFN_toxic: N terminal domain of HypF (HypF-N), [U-100% 13C; U-100% 15N], 50 uM; D2O, [U-100% 2H], 10%; DTT 2 mM; sodium acetate 50 mM; TFE 12%

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCHypFN_toxicisotropicsample_conditions_1
3D CBCA(CO)NHHypFN_toxicisotropicsample_conditions_1
3D HNCACBHypFN_toxicisotropicsample_conditions_1
3D HNCOHypFN_toxicisotropicsample_conditions_1
3D HN(CO)CAHypFN_toxicisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN_Analysis v2.4, W.F. Vranken, W Boucher, T.J. Stevens, R.H. Fogh, A Pajon, M. Llinas, E.L. Ulrich, J.L. Markley, J Ionides and E.D. Laue - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks