BMRB Entry 27195

Name: NMR Chemical Shift Assignments of Frataxin protein from Chaetomium thermophilum
Published: 2017-11-16

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27195

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Chemical Shift Assignments of Frataxin protein from Chaetomium thermophilum

Deposition date:

2017-07-31

Original release date:

2017-11-16

Authors:

Rasheed, Masooma; Yan, Robert; Kelly, Geoff; Pastore, Annalisa

Citation:

Citation: Rasheed, Masooma; Yan, Robert; Kelly, Geoff; Pastore, Annalisa. "Chemical shift assignment of a thermophile frataxin." Biomol. NMR Assign. 12, 113-116 (2018).
PubMed: 29090418

Assembly members:

Assembly members:
Ct-Frataxin, polymer, 152 residues, 14310 Da.

Natural source:

Natural source: Common Name: Chaetomium thermophilum Taxonomy ID: 209285 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Chaetomium Thermophilum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ct-Frataxin: MADITTAEYHRLADEYLDAL LSRLEELQDEREDVDVEYQS GVLTLNMGPEVGTYVINKQP PNKQIWLSSPKSGPKRYDYV ITGEGQNEKQDTAVGEWVYL RDGSTLNQLLLEEIGVDLNV PVSQD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	262
15N chemical shifts	119
1H chemical shifts	119

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Frataxin	1

Entities:

Entity 1, Frataxin 152 residues - 14310 Da.

1

MET

ALA

ASP

ILE

THR

ALA

GLU

TYR

HIS

2

ARG

LEU

ALA

ASP

GLU

TYR

LEU

ASP

ALA

LEU

3

LEU

SER

ARG

LEU

GLU

LEU

GLN

ASP

GLU

4

ARG

GLU

ASP

VAL

ASP

VAL

GLU

TYR

GLN

SER

5

GLY

VAL

LEU

THR

LEU

ASN

MET

GLY

PRO

GLU

6

VAL

GLY

THR

TYR

VAL

ILE

ASN

LYS

GLN

PRO

7

PRO

ASN

LYS

GLN

ILE

TRP

LEU

SER

PRO

8

LYS

SER

GLY

PRO

LYS

ARG

TYR

ASP

TYR

VAL

9

ILE

THR

GLY

GLU

GLY

GLN

ASN

GLU

LYS

GLN

10

ASP

THR

ALA

VAL

GLY

GLU

TRP

VAL

TYR

LEU

11

ARG

ASP

GLY

SER

THR

LEU

ASN

GLN

LEU

12

LEU

GLU

ILE

GLY

VAL

ASP

LEU

ASN

VAL

13

PRO

VAL

SER

GLN

ASP

Samples:

sample_1: Ct-Frataxin, [U-100% 15N], 1 mM; Ct-Frataxin, [U-100% 13C], 1 mM

sample_conditions_1: ionic strength: 4.20 M; pH: 7.4; pressure: 1 atm; temperature: 310.02 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, CCPN - chemical shift assignment, processing

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks