Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27206
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Citation: Citron, Y Rose; Fagerstrom, Carey; Keszthelyi, Bettina; Huang, Bo; Rusan, Nasser; Kelly, Mark; Agard, David. "The centrosomin CM2 domain is a multi-functional binding domain with distinct cell cycle roles" PLoS ONE 13, e0190530-e0190530 (2018).
PubMed: 29315319
Assembly members:
CM2, polymer, 63 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET51a
Entity Sequences (FASTA):
CM2: GPGSENAELRRKLIRTKRAF
EDTYEKLRMANKAKAQVEKD
IKNQILKTHNVLRNVRSNME
NEL
Data type | Count |
13C chemical shifts | 179 |
15N chemical shifts | 61 |
1H chemical shifts | 107 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Cm2 | 1 |
Entity 1, Cm2 63 residues - Formula weight is not available
Residues 1-4 are non-native residues leftover from the protease cleavage site. Chemical shifts from residues 1 and 2 are not identified.
1 | GLY | PRO | GLY | SER | GLU | ASN | ALA | GLU | LEU | ARG | ||||
2 | ARG | LYS | LEU | ILE | ARG | THR | LYS | ARG | ALA | PHE | ||||
3 | GLU | ASP | THR | TYR | GLU | LYS | LEU | ARG | MET | ALA | ||||
4 | ASN | LYS | ALA | LYS | ALA | GLN | VAL | GLU | LYS | ASP | ||||
5 | ILE | LYS | ASN | GLN | ILE | LEU | LYS | THR | HIS | ASN | ||||
6 | VAL | LEU | ARG | ASN | VAL | ARG | SER | ASN | MET | GLU | ||||
7 | ASN | GLU | LEU |
sample_1: sodium chloride 150 mM; Trizma 10 mM; sodium azide .02%; ammonium sulfate, [U-100% 15N], 1.25 g/L; glucose, [U-100% 13C], 2 g/L; Centrosomin, [U-100% 13C;U-100% 15N], 715 uM
sample_conditions_1: ionic strength: 0.15 M; pH: 7.2; pressure: 1 atm; temperature: 300 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D CBCANH | sample_1 | isotropic | sample_conditions_1 |
3D hNcaNH | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CCPNMR, CCPN - chemical shift assignment, peak picking
TOPSPIN, Bruker Biospin - collection
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks