BMRB Entry 27207

Name: Gcn4 tAD 1-134
Published: 2018-03-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27207

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Gcn4 tAD 1-134

Deposition date:

2017-08-07

Original release date:

2018-03-15

Authors:

Tuttle, Lisa; Pacheco, Derek; Warfield, Linda; Klevit, Rachel; Hahn, Steven

Citation:

Citation: Tuttle, Lisa; Pacheco, Derek; Warfield, Linda; Luo, Jie; Ranish, Jeff; Hahn, Steven; Klevit, Rachel. "Gcn4-Mediator Specificity Is Mediated by a Large and Dynamic Fuzzy Protein-Protein Complex." Cell Rep. 22, 3251-3264 (2018).
PubMed: 29562181

Assembly members:

Assembly members:
Gcn4_tAD, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDP15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Gcn4_tAD: MSEYQPSLFALNPMGFSPLD GSKSTNENVSASTSTAKPMV GQLIFDKFIKTEEDPIIKQD TPSNLDFDFALPQTATAPDA KTVLPIPELDDAVVESFFSS STDSTPMFEYENLEDNSKEW TSLFDNDIPVTTDD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	376
15N chemical shifts	119
1H chemical shifts	119

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Gcn4 tAD	1

Entities:

Entity 1, Gcn4 tAD 134 residues - Formula weight is not available

1

MET

SER

GLU

TYR

GLN

PRO

SER

LEU

PHE

ALA

2

LEU

ASN

PRO

MET

GLY

PHE

SER

PRO

LEU

ASP

3

GLY

SER

LYS

SER

THR

ASN

GLU

ASN

VAL

SER

4

ALA

SER

THR

SER

THR

ALA

LYS

PRO

MET

VAL

5

GLY

GLN

LEU

ILE

PHE

ASP

LYS

PHE

ILE

LYS

6

THR

GLU

ASP

PRO

ILE

LYS

GLN

ASP

7

THR

PRO

SER

ASN

LEU

ASP

PHE

ASP

PHE

ALA

8

LEU

PRO

GLN

THR

ALA

THR

ALA

PRO

ASP

ALA

9

LYS

THR

VAL

LEU

PRO

ILE

PRO

GLU

LEU

ASP

10

ASP

ALA

VAL

GLU

SER

PHE

SER

11

SER

THR

ASP

SER

THR

PRO

MET

PHE

GLU

TYR

12

GLU

ASN

LEU

GLU

ASP

ASN

SER

LYS

GLU

TRP

13

THR

SER

LEU

PHE

ASP

ASN

ASP

ILE

PRO

VAL

14

THR

ASP

Samples:

sample_1: Gcn4 tAD, [U-100% 13C; U-100% 15N], 500 uM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 5 mM; PMSF 0.1 mM; EDTA 0.1 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks