BMRB Entry 27268

Title:
Backbone chemical shifts for the complex between WASp interacting protein (WIP) and the EVH1 domain of WASp
Deposition date:
2017-10-01
Original release date:
2017-12-14
Authors:
Halle-Bikovski, Adi; Shaked, Hadassa; Chill, Jordan
Citation:

Citation: Halle-Bikovski, Adi; Fried, Sophia; Rozentur-Shkop, Eva; Biber, Guy; Shaked, Hadassa; Joseph, Noah; Baarda-Saad, Mira; Chill, Jordan. "New Structural Insights into Formation of the Key Actin Regulating WIP-WASp Complex Determined by NMR and Molecular Imaging"  ACS Chem. Biol. 13, 100-109 (2018).
PubMed: 29215267

Assembly members:

Assembly members:
WIP(442-492), polymer, 51 residues, Formula weight is not available
WASp(20-158), polymer, 139 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts526
15N chemical shifts167
1H chemical shifts563

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WIP1
2WASp2

Entities:

Entity 1, WIP 51 residues - Formula weight is not available

1   GLNASPSERPROCYSGLUASPGLUTRPGLU
2   SERARGPHETYRPHEHISPROILESERASP
3   LEUPROPROPROGLUPROTYRVALGLNTHR
4   THRLYSSERTYRPROSERLYSLEUALAARG
5   ASNGLUSERARGSERGLYSERASNARGARG
6   GLU

Entity 2, WASp 139 residues - Formula weight is not available

1   GLNASNILEPROSERTHRLEULEUGLNASP
2   HISGLUASNGLNARGLEUPHEGLUMETLEU
3   GLYARGLYSCYSLEUTHRLEUALATHRALA
4   VALVALGLNLEUTYRLEUALALEUPROPRO
5   GLYALAGLUHISTRPTHRLYSGLUHISCYS
6   GLYALAVALCYSPHEVALLYSASPASNPRO
7   GLNLYSSERTYRPHEILEARGLEUTYRGLY
8   LEUGLNALAGLYARGLEULEUTRPGLUGLN
9   GLULEUTYRSERGLNLEUVALTYRSERTHR
10   PROTHRPROPHEPHEHISTHRPHEALAGLY
11   ASPASPCYSGLNALAGLYLEUASNPHEALA
12   ASPGLUASPGLUALAGLNALAPHEARGALA
13   LEUVALGLNGLULYSILEGLNLYSARGASN
14   GLNARGGLNSERGLYASPARGARGGLN

Samples:

sample_1: WIP(442-492), [U-98% 13C; U-98% 15N], 0.5 mM; WASp(20-158), [U-98% 13C; U-98% 15N], 0.5 mM; D2O, [U-99% 2H], 7%; sodium chloride 50 mM; sodium phosphate 20 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.2, Bruker Biospin - collection

NMR spectrometers:

  • Bruker DRX 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks