BMRB Entry 27296

Title:
Methyl-methionine resonance assignments for Turkey beta-1 adrenergic receptor, Delta5-L190M mutant, bound to nanobody Nb6B9, without orthosteric ligand.
Deposition date:
2017-10-26
Original release date:
2018-01-29
Authors:
Solt, Andras; Bostock, Mark; Shrestha, Binesh; Kumar, Prashant; Warne, Tony; Tate, Christopher; Nietlispach, Daniel
Citation:

Citation: Solt, Andras; Bostock, Mark; Shrestha, Binesh; Kumar, Prashant; Warne, Tony; Tate, Christopher; Nietlispach, Daniel. "Insight into partial agonism by observing multiple equilibria for ligand-bound and Gs-mimetic nanobody-bound beta1-adrenergic receptor"  Nat. Commun. 8, 1795-1795 (2017).
PubMed: 29176642

Assembly members:

Assembly members:
Turkey_B1AR_D5_L190M, polymer, 317 residues, Formula weight is not available
Nb6B9, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Turkey   Taxonomy ID: 9103   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Meleagris gallopavo

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Spodoptera frugiperda   Vector: pBacPAK8

Data typeCount
13C chemical shifts14
1H chemical shifts42

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Turkey beta-1 adrenergic receptor, Delta5-L190M mutant, conformer 11
2Turkey beta-1 adrenergic receptor, Delta5-L190M mutant, conformer 21
3Nanobody Nb6B92

Entities:

Entity 1, Turkey beta-1 adrenergic receptor, Delta5-L190M mutant, conformer 1 317 residues - Formula weight is not available

1   METGLYALAGLULEULEUSERGLNGLNTRP
2   GLUALAGLYLEUSERLEULEULEUALALEU
3   VALVALLEULEUILEVALALAGLYASNVAL
4   LEUVALILEALAALAILEGLYSERTHRGLN
5   ARGLEUGLNTHRLEUTHRASNLEUPHEILE
6   THRSERLEUALACYSALAASPLEUVALMET
7   GLYLEULEUVALVALPROPHEGLYALATHR
8   LEUVALVALARGGLYTHRTRPLEUTRPGLY
9   SERPHELEUCYSGLULEUTRPTHRSERLEU
10   ASPVALLEUCYSVALTHRALASERILETRP
11   THRLEUCYSVALILEALAILEASPARGTYR
12   LEUALAILETHRSERPROPHEARGTYRGLN
13   SERLEUMETTHRARGALAARGALALYSVAL
14   ILEILECYSTHRVALTRPALAILESERALA
15   LEUVALSERPHELEUPROILEMETLEUHIS
16   TRPTRPARGASPGLUASPPROGLNALAMET
17   LYSCYSTYRGLNASPPROGLYCYSCYSASP
18   PHEVALTHRASNARGALATYRALAILEALA
19   SERSERILEILESERPHETYRILEPROLEU
20   LEUILEMETILEPHEVALTYRLEUARGVAL
21   TYRARGGLUALALYSGLUGLNILEARGLYS
22   ILEASPARGALASERLYSARGLYSTHRSER
23   ARGVALALAALAMETARGGLUHISLYSALA
24   LEULYSTHRLEUGLYILEILEMETGLYVAL
25   PHETHRLEUCYSTRPLEUPROPHEPHELEU
26   VALASNILEVALASNVALPHEASNARGASP
27   LEUVALPROASPTRPLEUPHEVALALAPHE
28   ASNTRPLEUGLYTYRALAASNSERALAALA
29   ASNPROILEILETYRCYSARGSERPROASP
30   PHEARGLYSALAPHELYSARGLEULEUALA
31   PHEPROARGLYSALAASPARGARGLEUHIS
32   HISHISHISHISHISHISHIS

Entity 2, Nanobody Nb6B9 132 residues - Formula weight is not available

1   HISHISHISHISHISHISLEUVALPROARG
2   GLYSERGLNVALGLNLEUGLNGLUSERGLY
3   GLYGLYLEUVALGLNALAGLYGLYSERLEU
4   ARGLEUSERCYSALAALASERGLYSERILE
5   PHEALALEUASNILEMETGLYTRPTYRARG
6   GLNALAPROGLYLYSGLNARGGLULEUVAL
7   ALAALAILEHISSERGLYGLYTHRTHRASN
8   TYRALAASNSERVALLYSGLYARGPHETHR
9   ILESERARGASPASNALAALAASNTHRVAL
10   TYRLEUGLNMETASNSERLEULYSPROGLU
11   ASPTHRALAVALTYRTYRCYSASNVALLYS
12   ASPPHEGLYALAILEILETYRASPTYRASP
13   TYRTRPGLYGLNGLYTHRGLNVALTHRVAL
14   SERSER

Samples:

L190M-Nb6B9: Turkey_B1AR_D5_L190M, 13Ce-Met, 90 uM; Nb6B9 350 uM; NaCl 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 8.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C SOFAST-HMQCL190M-Nb6B9isotropicsample_conditions_1

Software:

CCPN_Analysis v2.4.2, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz